幽门螺杆菌基因重组尿素酶亚单位免疫预防作用的实验研究  

Evaluation of immune prophylaxis of recombinant urease subunit of Helicobacter pylori

作　　者：施理[1] 王继德[1] 陈烨[1] 张振书[1] 张亚历[1] 张万岱[1] 周殿元[1] 

机构地区：[1]第一军医大学南方医院全军消化病研究所,广州510515

出　　处：《中华医学杂志》2000年第11期811-815,共5页National Medical Journal of China

基　　金：国家自然科学基金!资助项目 (395 70 334)

摘　　要：目的　评价幽门螺杆菌 (Hp)基因重组尿素酶A、B亚单位 (rUreA、rUreB)的免疫预防作用。方法　将BALB/C小鼠分为如下 7组 :阴性对照组 (10只 )、霍乱毒素B亚单位 (CTB)对照组 (10只 )、rUreA对照组 (10只 )、rUreB对照组 (10只 )、rUreA +CTB实验组 (30只 )、rUreB +CTB实验组 (30只 )、Hp超声粉碎物 +CTB实验组 (30只 ) ,胃内分别接种如下物质 :15 0ml磷酸盐缓冲液、5 μgCTB、5 0μgrUreA、5 0 μgrUreB、5 0 μgrUreA +CTB、5 μgrUreB +CTB、40 0 μgHp超声粉碎物 + 5 μgCTB ;2周后胃内接种Hp(10 7～ 10 8CPU/次 ) ;4周、8周、12周后处死小鼠 ,用胃组织切片Giemsa染色、显微镜下检查Hp的方法评价免疫预防作用 ;另外 ,rUreA +CTB毒副作用组 (10只 )、rUreB +CTB毒副作用组 (10只 )小鼠 ,只接种rUreA +CTB或rUreB +CTB ,而不感染Hp ,12周后处死 ,用胃组织切片HE染色、显微镜下观察炎症细胞浸润的方法评价毒副作用。结果　给小鼠胃内单独接种CTB或rUreA或rUreB ,可使免疫小鼠胃粘膜Hp感染数目明显减少。CTB能增强rUreA、rUreB免疫保护作用。接种rUreA +CTB较接种rUreB +CTB或Hp超声粉碎物 +CTB ,免疫保护作用产生的较晚。接种rUreA +CTB或rUreB +CTB或Hp超声粉碎物Objective To evaluate the immune prophylaxis of recombinant urease subunit A or B (rUreA or rUreB). Methods The BALB/C mice were divided into seven groups: negative control group, control group with CTB, control group with rUreA, control group with rUreB, experimental group with rUreA+CTB and experimental group with rUreB+CTB, experimental group with sonicate+CTB, which were gastrically inoculated with 150 ml PBS, 5 μg CTB, 50 μg rUreA, 50 μg rUreB, 50 μg rUreA+CTB, 5 μg rUreB+CTB and 400 μg sonicate+5 μg CTB, respectively. Two weeks later, the mice were gastrically infected with H.pylori (10 7～10 8 CPU per time). 4, 8 and 12 weeks later, the mice were killed and the immune protection of PBS, CTB, rUreA, rUreB, rUreA+CTB, rUreB+CTB and sonicate+CTB were evaluated by the presence of H.pylori in gastric tissue stained with Giemsa by microscopy. The mice in the side effecting groups of rUreA+CTB and rUreB+CTB were not infected with H.pylori . 12 weeks later, the mice in the two groups were killed, and the side effects of rUreA+CTB and rUreB+CTB were evaluated by the infiltration of inflammation cells in gastric tissue stained with HE by microscopy. Results The mice gastrically inoculated with CTB, rUreA or rUreB had significantly less H.pylori. The immune protective effect of rUreA, rUreB or sonicate of H.pylori combined with CTB was stronger than that of rUreA, rUreB or sonicate of H.pylori alone. The immune protective effect of rUreA+CTB occurred later than that of rUreB+CTB or sonicate of H.pylori +CTB. The mice immunized with rUreA+CTB, rUreB+CTB or sonicate of H.pylori +CTB were not completely protected against H.pylori infection. The mice immunized with rUreA+CTB, rUreB+CTB or sonicate of H.pylori +CTB were less infected with H.pylori , and had mild inflammation of gastric tissue. Conclusion Mice immunized with rUreA+CTB, rUreB+CTB or sonicate of H.pylori +CTB could not be completely protected against H.pylori infection and have alleviated inflammat

关 键 词：幽门螺杆菌 基因重组 尿素酶 亚单位 免疫预防 

分 类 号：R392[医药卫生—免疫学]