健那绿作光谱探针共振瑞利散射法测定藻酸钠  

Determination of Sodium Alignate with Janus Green as a Probe by Resonance Rayleigh Scattering Spectrometry

作　　者：韩权[1] 田丽[2] 霍燕燕[1] 刘荣利[1] 杨龙虎[1] 杨晓慧[1] 

机构地区：[1]西安文理学院化学与化学工程学院,陕西西安710065 [2]陕西省疾病预防控制中心理化检验中心,陕西西安710054

出　　处：《分析科学学报》2013年第4期473-477,共5页Journal of Analytical Science

基　　金：陕西省自然科学基础研究计划(No.2010JM2018);西安市科技计划(No.CXY1134WL38)

摘　　要：本文提出了共振瑞利散射法测定藻酸钠的新方法。研究发现在pH=4.0的Britton-Robinson缓冲溶液中,藻酸钠或健那绿单独存在时共振瑞利散射(RRS)强度非常弱,当两者反应形成复合物时,RRS大大增强并产生新的RRS光谱,其最大RRS峰位于560nm,另在328nm和397nm处产生两个强度较低的散射峰。在560nm处,藻酸钠的浓度在0.015～1.0μg/mL范围内与RRS强度有良好的线性关系,检出限(3σ/k)为5ng/mL。方法灵敏度高,选择性好,可用于面条和海带提取液中的藻酸钠测定。A novel method for the determination of sodium alignate（SA） based on resonance Rayleigh scattering（RRS） has been developed. In pH 3.0-8.0 Britton-Robinson buffer solution,the RRS intensity of sodium alignate（SA） or Janus Green（JG） alone was very weak while it was enhanced greatly and gave a new RRS spectrum when they combined with each other to form a new product. The maximum resonance scattering peak was located at 560 nm,and other two less strong peaks were located at 328 and a97 nm,respectively. The intensity of RRS was directly proportional to the concentration of SA in the range of 0.015-1.0 μg/mL at 560 nm. The detection limit（3a/k） for SA was found to be 5 ng/mL. The method was highly sensitive and selective,and has been used for the determination of sodium alignate in noodle and kelp extracts with satisfactory results.

关 键 词：共振瑞利散射 健那绿 藻酸钠 

分 类 号：O657.3[理学—分析化学]