方波伏安法研究[Ru(bpy)_2dppz]^(2+)与DNA的相互作用  

Square Wave Voltammetric Studies of the Interaction of[Ru(bpy)_2dppz]^(2+) with DNA

作　　者：熊岳[1] 康天放[1] 鲁理平[1] 

机构地区：[1]北京工业大学环境与能源工程学院,北京100022

出　　处：《分析科学学报》2013年第4期493-496,共4页Journal of Analytical Science

基　　金：国家自然科学基金(No.21005005);北京市教委科技发展基金重点项目(No.KZ201110005006);北京市自然科学基金(No.8102009)

摘　　要：合成了含有嵌入配体二吡啶并[3,2-a:2′,3′-c]-吩嗪(dppz)的钌配合物二联吡啶二吡啶并[3,2-a;2′,3′-c]吩嗪钌([Ru(bpy)2dppz]2+),并对其结构和物理化学性质进行了表征。采用方波伏安法研究了[Ru(bpy)2dppz]2+与天然双链小牛胸腺DNA的相互作用,实验结果表明,[Ru(bpy)2dppz]2+配合物的嵌入配体会嵌入DNA的碱基对中,与DNA结合形成体积较大的"金属配合物-DNA"联合体,该联合体在电解质溶液中扩散速度较慢,导致溶液中游离的钌配合物分子减少,峰电流信号降低。计算得到[Ru(bpy)2dppz]2+与DNA的结合常数Ka=1.7×105 L/mol,结合位点n=0.84。The ruthenium complex with DPPZ （dipyrido[-3, 2-a： 2＇, 3＇-c]-phenazine） as a ligand was prepared. TheERu（bpy）zdpp2]2＋ （bpy=2,2-bipyridine, dppz=dipyrido[3,2-a：2＇,3＇-c] phenazine） complex was characterized by UV-Vis,IH NMR,fluorescenee spectra and electrochemical analysis and was found to exhibit the expected propellerqike structure with significant intermolecular ~r-stacking interactions. The binding interaction of [Ru （bpy）a dppz]2＋ with calf-thymus deoxyribonucleic acid （DNA） was studied by square wave voltammetry. The result showed that metallo-intercalators could intercalate into the double helix of DNA,and formed a ＂metallo-intercalators-DNA＂ complex. Due to the slow diffusion of the complex in the solution, anodic peak current decreased. The binding parameters, including the binding constant （K.） and the binding-site size （n）, were obtained simultaneously by the analysis of bound and free [Ru（bpy）2dppz]2＋ concentration corresponding to the limits of slow and fast binding kinetics compared to the experimental timescale. The binding constant（Ka） was 1.7×10s L/mol, and the calculated binding-site size（n） was 0.84 base pair,which was consistent with the reported values.

关 键 词：DNA [Ru(bpy)2dppz]2+ 伏安法 结合常数 

分 类 号：O657.1[理学—分析化学]