翘鳞伞菌丝体生长培养研究  

Cultivation of Pholiota squarrosa Mycelium

作　　者：付振艳[1] 吴金平[1] 苟小清[1] 王晓军[1] 

机构地区：[1]中国科学院新疆理化技术研究所,乌鲁木齐830011

出　　处：《西北农业学报》2013年第8期78-82,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：新疆维吾尔自治区自然基金会项目(2011211B507);中国科学院西部博士资助项目(XBBS200914)

摘　　要：为了提高生产效率、缩短翘鳞伞的生长周期,对翘鳞伞菌丝体的生长培养条件进行优化。单因素试验以及Plackett-Burman试验结果表明:翘鳞伞菌丝体生长的最适碳源为麦芽糖,最适氮源为酵母粉,最适pH为5,最适温度为23℃;影响翘鳞伞生长的主要因素为麦芽糖、酵母粉和K2HPO4。通过最陡爬坡试验和正交试验确定翘鳞伞菌丝生长的最优培养基为:35g/L麦芽糖+1.5g/L酵母粉+0.5g/L K2HPO4+0.5g/L KH2PO4+0.5g/L MgSO4。在最佳培养条件下,菌丝体菌落直径由6.11cm增加到7.41cm。The cultivation conditions of Pholiota squarrosa mycelium were optimized in order to enhance product efficiency and shorten cultivation cycle.The one-factor experimental design and Plackett-Burman design showed that the optimum carbon source was maltose,the optimum nitrogen source was yeast,the optimum pH was 5,and that the optimum temperature was 23 ℃.What was more,the main factors influencing mycelium growth were maltose,yeast and K2HPO4.The optimum medium for Pholiota squarrosa mycelium growth was identified as 35g/L maltose＋1.5g/L yeast＋0.5 g/L K2HPO4＋0.5g/L KH2PO4＋0.5g/L MgSO4by the experiments of the steepest ascent and orthogonal designs.On the optimum medium the colony diameter was increased from 6.11cm to 7.41cm.

关 键 词：翘鳞伞 生长条件 PLACKETT-BURMAN设计 正交设计 

分 类 号：S646[农业科学—蔬菜学]