HPLC法测定卷柏及其炮制品中穗花杉双黄酮的含量  

Determination of Amentoflavone Contents in Selaginella tamariscina and Different Processed Products by HPLC

作　　者：吴彩霞[1,2] 杨宇婷[1] 康文艺[1] 

机构地区：[1]河南大学中药研究所,开封475004 [2]河南省疾病控制中心,郑州450003

出　　处：《天然产物研究与开发》2013年第8期1089-1091,1129,共4页Natural Product Research and Development

基　　金：河南省卫生厅科技创新人才工程(4049);河南省科技厅重点项目(122102310053)

摘　　要：采用HPLC方法测定卷柏及炮制品中穗花杉双黄酮的含量。方法:Shim-pack VP-ODS柱(4.6 mm×250mm,5μm);流动相:甲醇-0.1%磷酸水(65∶35);流速:0.8 mL/min;检测波长:337 nm;柱温:25℃。实验结果表明,卷柏生品中穗花杉双黄酮的含量为0.84%,焦卷柏为1.07%,卷柏炭为0.53%。不同的炮制方法对穗花杉双黄酮的含量产生不同的影响。The objective of this study is to determine the concentrations of amentoflavone in Selaginella tamacina and different processed products by high performance liquid chromatography （HPLC）. The HPLC separation was performed on Shim-pack VP-ODSoolunm （4.6 mm × 250 mm,5μm） and detected with DAD at the wavelength 337 ran. The isocratic elution was carried out with methanol - 0.1% phosphoric acid in water （65： 35） as the mobile phase at a flow rate of 0.8 mL/mln at 25℃. The results indicated that the concentrations of amentoflavone detected in S. tamariscina,fried S. Tamariscina and S. tamariscina coke were 0.84% ,1.07% and 0.53% respectively. Different processing methods caused the different concentrations of amentoflavone in these samples.

关 键 词：HPLC 卷柏 炮制 穗花杉双黄酮 

分 类 号：Q946.91[生物学—植物学] R284.1[医药卫生—中药学]