神经再生条件液对运动神经元细胞活性的影响  

NEUROBIOLOGICAL EFFECT OF NERVE REGENERATION CONDITIONED FLUID ON MOTONEURON

作　　者：平萍[1] 李青峰[1] 干季良[1] 张涤生[1] 

机构地区：[1]上海第二医科大学附属第九人民医院整复外科,上海200011

出　　处：《中国修复重建外科杂志》2000年第6期325-327,共3页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：中国博士后科学基金!资助项目 (1995 -1);上海市卫生局科研基金!资助项目 (97BR0 13)

摘　　要：目的　比较源于运动及感觉神经的再生条件液 (NRCF)以及碱性成纤维细胞生长因子 (b FGF)对体外分散培养的 SD大鼠运动神经元的生物学效应 ,探讨周围神经再生趋化特异性产生的机制。方法　建立运动及感觉神经再生室模型 ,收集术后 7天的运动及感觉神经再生条件液 (MD- NRCF,SD- NRCF) ,将两者以及 b FGF分别加入分散培养的 SD大鼠运动神经元的 DMEM无血清培养基中 ,在倒置相差显微镜下观察并摄片 ,运用细胞图像分析系统测量各组细胞的胞体面积及最长突起长度 ;并采用 MTT法检测各组神经元的细胞活性。结果　实验组运动神经元的胞体面积、最长突起长度及 OD值均大于对照组 ;其中 MD- NRCF组细胞的最长突起长度及 OD值大于 SD- NRCF组和 b FGF组。结论　 MD- NRCF对于运动神经元的促突起生长作用及神经生物学活性优于 SD-NRCF及 b FGF。Objective To explore the mechanism of tissue specificity of neurotropism in peripheral nerve regeneration, we investigated the biological characteristics of the nerve regeneration conditioned fluids(NRCF) on motoneuron of SD rats cultured in vitro.Methods Silicon chambers were sutured respectively to the distal stumps of motorial branch of femoral nerve and saphenous nerve to collect NRCF, namely MD-NRCF and SD-NRCF. The rats cortex motoneuron were divided into 4 groups and cocultured with MD-NRCF, SD-NRCF, b-FGF and serum-free medium respectively. The cultured cells were photoed under phase-constrast microscope, their longest neurites and cell-body areas were measured by cell image processing computer system. MTT automated colorimetric microassay was also adopted to quantify the activation of cultured motoneurons in each group.Results Cells of MD-NRCF group had longer neurites than those of the other three groups, and their activation was also superior to those of the other groups.Conclusion The results suggest that MD-NRCF has more significantly neurite-promoting and neurobiological effects on motoneuron than SD-NRCF and b-FGF.

关 键 词：运动神经元 周围神经再生 神经再生条件液 

分 类 号：R651.3[医药卫生—外科学]