连续传代人胚骨骼肌成肌细胞生物学特性研究  

BIOLOGICAL CHARACTERISTICS OF CONTINUOUSLY SUBCULTURED HUMAN EMBRYONIC SKELETAL MYOBLASTS

作　　者：岑石强[1] 杨志明[1] 解慧琪[1] 李胜富[2] 

机构地区：[1]华西医科大学附属第一医院骨科,成都610041 [2]华西医科大学附属第一医院移植免疫实验室,成都610041

出　　处：《中国修复重建外科杂志》2000年第6期372-376,共5页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家自然科学基金!资助项目 (39870 2 0 2 );国家重点基础研究发展规划! (973):组织工程的基本科学问题(G19990 5 430 8);美国纽约中

摘　　要：目的　探讨成肌细胞连续传代能力 ,选择适宜肌组织工程研究的成肌细胞。方法　常规传代培养人胚骨骼肌成肌细胞 ,以生长曲线、融合率分别观察细胞增殖、分化能力 ,探讨成纤维细胞沾染对传代细胞的影响。结果　第 6代以内细胞成纤维细胞沾染轻 ,主要表现出成肌细胞的生长特性 ,增殖较旺盛 ,分化能力高。第 8代～第 16代细胞成纤维细胞沾染重 ,表现出成纤维细胞的生长特性 ,增殖速度明显加快但分化能力低。第 2 0代细胞退变明显 ,细胞增殖与分化能力均丧失。结论　第 6代以内细胞生长特性以成肌细胞为主 ,增殖能力强 ,肌小管形成率高 ,适宜于组织工程研究。Objective To investigate the biological characteristics of continuously subcultured human embryonic skeletal myoblasts, and choose the optimal seeding cells for muscle tissue engineering. Methods Human embryonic skeletal myoblasts were subcultured in vitro. The growth curve, rate of myotube formation(RMF) were used to evaluate the proliferative and differentiation ability of myoblasts, and to investigate the influence of fibroblasts contamination on myoblasts. Results The beginning 6 passages of myoblasts showed strong proliferative and differentiation ability. From the 8th to 20th passage, the rate of fibroblasts contamination was increased, it mainly showed the growth characteristics of fibroblasts with increased proliferation and low differentiation. After subcultured to the 20th passage, the degeneration of myoblasts was obvious. Conclusion The myoblasts within 6 passages should be used as the seeding cells of muscle tissue engineering because of strong proliferative ability and high rate of myotube formation.

关 键 词：胚骨骼肌 成肌细胞 生物学特征 传代培养 

分 类 号：R329.26[医药卫生—人体解剖和组织胚胎学]