肾小管间质纤维化中转化生长因子β1的表达特征  

The expression of transforming growth factor β1 in tubulointerstitial fibrosis

作　　者：孙铭柱[1] 徐曼[2] 邹万忠[3] 

机构地区：[1]解放军第二炮兵总医院病理科 [2]重庆医科大学病理系 [3]北京医科大学病理系,100083

出　　处：《中华肾脏病杂志》2000年第5期291-295,共5页Chinese Journal of Nephrology

基　　金：国家自然科学基金!(39770293)

摘　　要：目的研究大鼠肾小管间质纤维化中转化生长因子β１（ＴＧＦ－β１）的表达特征。方法以腺嘌呤灌胃法建立大鼠肾小管间质纤维化实验模型，应用免疫组织化学（组化）、逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）、原位杂交及 Ｎｏｒｔｈｅｒｎ ｂｌｏｔ检测 ６只正常及３０只模型大鼠肾组织ＴＧＦ－β１蛋白及基因表达。结果６只正常大鼠肾组织ＴＧＦ－β１主要位于皮髓交界处肾小管上皮细胞，ＴＧＦ－β１免疫组化平均阳性表达率为（４．２±０ ．８）％；损伤的肾小管上皮细胞ＴＧＦ－β１表达增加，给药１～５周，３０只模型肾组织 ＴＧＦ－β１免疫组化平均阳性表达率分别为（８． ３± ０． ４）％、（９． ６± ０． ５）％、（ １１． ５± １． ０）％。（１６．６±０．８）％及（１７． ８ ±０．９）％。各实验组ＴＧＦ－β１平均阳性表达率均与正常对照组的差异有显著性意义（Ｐ＜０．０１）；实验组的ＴＧＦ－β１ｍＲＮＡ表达高于正常组，而且随着肾小管损伤和间质纤维化的日益加重，实验第５周ＴＧＦ－β１ ｍＲＮＡ表达量最高。结论 ＴＧＦ－β１在肾小管间质纤维化中主要由受损伤的肾小管上皮细胞产生，通过自分泌及旁分泌途径发挥促纤维化作用。Objective To investigate the expressive feature of transforming growth factor β1 (TGF-β1) in rats with tubulointerstitial fibrosis. Methods The tubulointerstitial fibrosis model was established in 30 Wistar rats by gastroperfusion of excessive adenine. The expressions of protein and mRNA of TGF-β1 were examined utilizing immunohistochemistry, in situ hybridization, reverse transcription polymerase chain reaction and Northern blot. Results The positive signals of TGF-β1 were mainly located in the tubular cells on the juxtamedullary regions and the average positive rate of TGF-β1 was (4. 2 ± 0. 8 ) % in 6 normal rats. The positive signals of TGF-β1 in injurious tubular cells were significantly higher than normal and the average positive rates of TGF-β1 were (8. 3 ± 0. 4)%, (9. 6 ± 0. 5 ) %, (11. 5 ± 1. 0) %, (16. 6 ± 0. 8)%, (17. 8 ± 0. 9)% respectively in experimental rats after gastroperfusion for one to five weeks. There was a significantly difference between the normal and fibrosis rats in the expression of TGF-β1 (P < 0. 01 ). The quantity of TGF-β1 mRNA in fibrosis rats was higher than that of the normal and the highest in the fifth week. Conclusion TGF-β1 is a promote growth factor in tubulointerstitial fibrosis, and is synthesized and secreted from injurious tubular cells and acts on interstitial cells.

关 键 词：转化生长因子Β1 肾小管间质纤维化 腺嘌呤 表达 

分 类 号：R692.6[医药卫生—泌尿科学]