反义寡核苷酸对间质性肾炎肾小管上皮细胞骨调素表达的调节  

Expression regulation of osteopontin by its antisense oligonucleotides in renal epithelial cells of interstitial nephritis

作　　者：陈永雄[1] 余学清[1] 孙玉玲[1] 陈伟英[1] 黄凌虹[1] 吕军[1] 范崇伦 尹培达[1] 

机构地区：[1]中山医科大学附属第一医院肾内科卫生部肾脏病临床研究重点实验室,广州510080

出　　处：《中华肾脏病杂志》2000年第5期296-299,共4页Chinese Journal of Nephrology

基　　金：卫生部科研基金!(98-1-090);广东省卫生厅基金!(A199814)

摘　　要：目的探讨骨调素（ＯＰＮ）反义寡核苷酸对间质性肾炎肾小管上皮细胞ＯＰＮ表达的影响。方法以一侧输尿管梗阻（ＵＵＯ）所致的间质性肾炎为研究模型，肾动脉内注射全硫代修饰的ＯＰＮ反义寡核苷酸，免疫组织化学双染及原位杂交技术检测ＯＰＮ的表达及巨噬细胞的浸润。结果ＯＰＮ及其ｍＲＮＡ在间质性肾炎的发展过程中表达明显升高，巨噬细胞的局部浸润均发生在过度表达ＯＰＮ的肾小管周围； ＯＰＮ反义寡核苷酸能显著抑制肾小管上皮细胞ＯＰＮ及其ｍＲＮＡ的表达，减少巨噬细胞的局部浸润。结论 ＯＰＮ反义寡核苷酸可能通过抑制 ＯＰＮ ｍＲＮＡ转录和翻译的方式抑制肾小管上皮细胞ＯＰＮ的表达。Objective To investigate the effect of antisense oligonucleotides on expression of osteopontin (OPN) in renal tubular epithelial cells of interstitial nephritis. Methods The expression of OPN and macrophage infiltration were detected in interstitial nephritis caused by unilateral ureteral obstruction (UUO) treated with OPN antisense oligonucleotides using double immunostaining and in situ hybridization. Results The expression of OPN and its mRNA was up-regulated in the development of interstitial nephritis, and macrophage infiltration occurred around tubules with up-regulated OPN expression. OPN antisense oligonucleotides inhibited the expression of OPN and its mRNA significantly in tubular cells and decreased local infiltration Of macrophage. Conclusion OPN antisense oligonucleotides can inhibit OPN expression by means of preventing OPN mRNA transcription and translation.

关 键 词：肾小管上皮细胞 骨调素 间质性肾炎 ASODN表达 

分 类 号：R692.33[医药卫生—泌尿科学]