高效液相色谱法单波长切换测定复方氯霉素醇溶液中水杨酸、氯霉素的含量  

High Performance Liquid Chromatography with Single Wavelength Switching in Determination of Contents of Salicylic Acid and Chloramphenicol in Compound Chloramphenicol Alcoholic Solution

作　　者：王敏[1] 张瑞麟[2] 王逢春[3] 李胜 

机构地区：[1]北京军区总医院263临床部医务处 [2]空军总医院药学部 [3]北京军区总医院医务处 [4]73089部队卫生队

出　　处：《解放军医药杂志》2013年第8期88-90,共3页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

摘　　要：目的建立高效液相色谱法,单波长切换测定复方氯霉素醇溶液中水杨酸、氯霉素的含量。方法色谱柱:Agilent Eclipse XDB-C18(4.6 mm×150 mm,5μm);柱温:30℃;流动相:甲醇-水(55∶45);流速:1.0 ml/min;检测波长:303 nm(0～4 min),278 nm(4～7 min)。结果水杨酸和氯霉素的线性范围为10.00～200.0 g/ml。平均回收率分别为99.90%、99.78%,相对标准偏差(RSD)分别为0.82%、0.99%(n=6)。结论该方法操作简单、准确可靠,可缩短复方氯霉素醇溶液样品的检测时间,提高水杨酸、氯霉素含量测定结果的准确性。Objective To establish a high performance liquid chromatography （HPLC） method with single wavelength switching in determination of contents of salicylic acid and chloramphenicol in compound ehloramphenicol al- coholic solution. Methods Agilent Eclipse XDB-C18（4.6 mm × 150 mm, 5 μm） of chromatographic column was used, and the column temperature was 30℃ ; the mobile phase was methanol-water （55： 45） , and the flow rate was 1.0 ml/ rain; the detection wavelengths were 303 nm （0-4 rain） and 278 nm （4-7 rain）. Results The linear ranges of salicylic acid and chloramphenicol was at 10.00-200.0 mg/ml, the average recoveries were 99.90% and 99.78% respectively, and relative standard deviation （RSD） were 0.82% and0.99% （n =6） respectively. ConcLusion The method is sim- ple, accurate and reliable, and it may shorten the detection time of Compound Chloramphenicol Alcoholic solution and improve the determination accuracy of Salicylic Acid and Chloramphenicol.

关 键 词：复方氯霉素醇 高效液相色谱法 单波长切换 含量测定 

分 类 号：R943.3[医药卫生—药剂学]