核黄素在掺铝硫化镉壳聚糖修饰电极上的电化学行为研究  

Study on Electrochemical Behavior of Riboflavin at the Glassy Carbon Electrode by CdS Doped with Al Ion and Chitosan

作　　者：赵强[1] 段德良[1] 陈朵[1] 杨晓云[1] 白慧萍[1] 王家强[1] 

机构地区：[1]云南大学化学科学与工程学院自然资源药物化学重点实验室,云南昆明650091

出　　处：《昆明学院学报》2013年第3期62-65,共4页Journal of Kunming University

基　　金：云南省应用基础研究基金资助项目(2011FZ011)

摘　　要：利用扫描电镜和交流阻抗法对以滴涂法制备的掺铝的硫化镉、壳聚糖修饰的玻碳电极进行纳米膜特性表征,并采用循环伏安法研究核黄素在其上的电化学行为.结果显示,在多种缓冲溶液中测试,发现在0.05 mol/L的邻苯二甲酸氢钾作为缓冲溶液时,电化学氧化还原峰形最好,峰电流较大.结果还表明,在修饰电极上的扩散系数D为5.08×10-4 cm2/s,反应速率常数k为5.36×10-7 mol/（L·s）.在实验优化选定的测试条件下,氧化峰电流与核黄素的浓度在5.00×10-6 ～4.50×10-5 mol/L范围内呈良好线性关系,检出限达2.55×10-6 mol/L,相对标准偏差（n=10）为4.25％,可用于核黄素含量的测定.By using the scanning electron microscopy（SEM） and the electrochemical impedance spectroscopy （EIS） construct surface features of the CdS doped with A1 ion （A1-CdS） and chitosan （Chi） solution and dropped on the surface of the glassy carbon electrode, and by using cyclic vohammetry （CV） studied the electrochemical behavior of riboflavin. The result showed that the shape of electro- chemical oxidation-reduction peak in Potassium biphthalate buffer is the best and the corresponding peak current is strongest among a variety of buffer solutions. The reversibility of the electrochemical behavior for riboflavin at A1-CdS-Chi modified glassy carbon electrode was found to be good. Based on the results that the property of kinetics including diffusion coeficient（D） and rate constant（k） were 5.08×10-4 cm2/s and 5.36×10-7 tool/（ L · s） respectively. On the optimum conditions. For oxidation peak （ - 0. 265 V）, its linear relationship being 5.00×10-6-4.50×10-5 mol/L with the detection limit of 2.55×10-6 mol/L. The RSD （ n = 10） of Riboflavin was 4.25%. The method was applied in the determination real sample of Riboflavin.

关 键 词：核黄素 掺铝硫化镉 壳聚糖 修饰电极 

分 类 号：O657.1[理学—分析化学] TQ460.72[理学—化学]