Effects of Two Drugs on Small Intestinal Epithelial Lymphocytes of Experimental Piglets with Spleen Deficiency  

作　　者：Ping SHEN Yanying ZHANG Guisheng GAO Qiumei SHI Guangping GAO Xinhua SHAO 

机构地区：[1]Key Laboratory of Preventive Veterinary Medicine of Hebei Province,Hebei Normal University of Science and Technology [2]Hebei Xinhua Animal Drug Co.,Ltd.

出　　处：《Agricultural Biotechnology》2013年第4期34-37,共4页农业生物技术（英文版）

基　　金：Supported by Major Project of the Ministry of Science and Technology of China(2012GB2A200044);Science and Technology Development Project of Shijiazhuang Municipal Science and Technology Bureau(08150132A-3);Scienceand Technology Development Project of Qinghuangdao City(201101A183)

摘　　要：[ Objective] This study aimed to investigate the effects of Guchang Cuzhang powder and Smecta on small intestinal epithelial lymphocytes of piglets with spleen deficiency and diarrhea induced by Reserpine. [ Method] Eighteen piglets with reserpine-induced spleen deficiency and diarrhea were selected and randomly divided into three groups, six per group, including Guchang Cuzhang powder group, Smeeta group and spleen deficiency control group. Piglets in two drug adnfinistration groups were treated for 7 d using Guchang Cuzhang powder and Smecta, respectively. On the 2nd d and 8th d after drug withdrawal, three piglets in each group were euthanized by jugular vein bleeding to death. Intestine segments in different groups were collected for paraffin embedding, section preparation and HE staining, to conduct histological observation and count the number of small intestinal epithelial lymphocytes. [ Result ] Compared with the spleen deficiency control group, the number of lymphocytes in small intestinal epithelium of piglets in two drug administration groups increased significantly; in addition, the number of lymphocytes in small intestinal epithelium of piglets in Guchang Cuzhang powder group was significantly higher than that in Smecta group. [ Conclusion ] This study provided theoretical basis for the clinical applieation of Guchang Cuzhang powder and Smecta.[ Objective] This study aimed to investigate the effects of Guchang Cuzhang powder and Smecta on small intestinal epithelial lymphocytes of piglets with spleen deficiency and diarrhea induced by Reserpine. [ Method] Eighteen piglets with reserpine-induced spleen deficiency and diarrhea were selected and randomly divided into three groups, six per group, including Guchang Cuzhang powder group, Smeeta group and spleen deficiency control group. Piglets in two drug adnfinistration groups were treated for 7 d using Guchang Cuzhang powder and Smecta, respectively. On the 2nd d and 8th d after drug withdrawal, three piglets in each group were euthanized by jugular vein bleeding to death. Intestine segments in different groups were collected for paraffin embedding, section preparation and HE staining, to conduct histological observation and count the number of small intestinal epithelial lymphocytes. [ Result ] Compared with the spleen deficiency control group, the number of lymphocytes in small intestinal epithelium of piglets in two drug administration groups increased significantly; in addition, the number of lymphocytes in small intestinal epithelium of piglets in Guchang Cuzhang powder group was significantly higher than that in Smecta group. [ Conclusion ] This study provided theoretical basis for the clinical applieation of Guchang Cuzhang powder and Smecta.

关 键 词：PIGLETS Guchang Cuzhang powder SMECTA Spleen deficiency Small intestine LYMPHOCYTE 

分 类 号：S858.28[农业科学—临床兽医学]