缺血引起陷窝蛋白-1在肺泡Ⅱ型上皮细胞定位的改变  

Ischemia Induced Caveolin-1 Moving from Cell Membrane to Lipid Droplets in Type Ⅱ Alveolar Epithelial Cell

作　　者：李凌海[1,2] 耿万明[1,2] 王子彤[1,2] 秦林[1,2] 张慧娜[3] 

机构地区：[1]北京市结核病胸部肿瘤研究所 [2]首都医科大学附属北京胸科医院,北京101149 [3]中国科学院生物物理研究所,北京100101

出　　处：《生物物理学报》2013年第6期414-421,共8页Acta Biophysica Sinica

基　　金：国家自然科学基金项目(81270932);北京市自然科学基金项目(5122028)~~

摘　　要：肺泡Ⅱ型上皮细胞在肺缺血病理过程中具有重要作用。为研究缺血对陷窝蛋白-1在肺泡Ⅱ型上皮细胞A549脂滴定位的影响,利用已经建立的脂滴纯化方法,纯化得到肺泡Ⅱ型上皮细胞A549的脂滴,并在脂滴上发现了陷窝蛋白-1。在A549细胞缺血模型中发现缺血可以导致陷窝蛋白-1从细胞膜移动到脂滴。人肺组织脂滴纯化实验也证实缺血可以刺激陷窝蛋白-1从细胞膜移动到脂滴。这一发现将为肺缺血机制的研究提供新的思路。Type II alveolar epithelial cells play an important role in ischemia of the lung. In this research, the authors studied the intracellular location of the caveolin-1 in type II alveolar epithelial cells under normal and ischemia status. They purified the lipid droplets from type II alveolar epithelial cell line A549. The results indicated that caveolin-1 was localized on plasma membrane as well as lipid droplets of alveolar epithelial cell, whereas ischemia stimulus induced caveolin-1 moving from cell membrane to lipid droplets in A549 cell line. In human lung tissue, They also observed the translocation of caveolin-1 from cell membrane to lipid droplets under ischemia status. These findings may promote new directions in future research concerning the mechanism of lung ischemia injury.

关 键 词：肺泡上皮细胞 脂滴 陷窝蛋白-1 

分 类 号：Q73[生物学—分子生物学]