鸡胚发育中免疫器官肥大细胞类胰蛋白酶的研究  

Tryptase of mast cells in immune organs of developing chicken embryos

作　　者：高登慧[1] 郭俊峰[1,2] 欧德渊[1] 姚红艳[1] 刘芳[1] 

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025 [2]贵州中医学院,贵州贵阳550002

出　　处：《细胞与分子免疫学杂志》2013年第10期1009-1011,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：贵州省科学技术基金资助项目[黔科合J字(2009)2132]

摘　　要：目的研究不同日龄鸡胚免疫器官中肥大细胞（MC）与类胰蛋白酶阳性细胞（TPC）的形态、分布及出现时间。方法取不同日龄鸡胚胸腺、脾脏和法氏囊，用Camoy’s液固定，阿尔新蓝-藏红O复染（AB／SO）法与免疫组织化学SABC法观察MC和TPC的分布。结果13～14日龄鸡胚的胸腺、法氏囊、脾脏中最早出现MC。随着日龄的增加，MC数量逐渐增多。16日龄鸡胚胸腺、脾脏和法氏囊组织中最早观察到TPC，且与MC的形态与分布十分相似，但出现的时间晚于MC。结论16日龄以后的鸡胚免疫器官中的MC内含有类胰蛋白酶，主要存在于结缔组织的MC中。Objective To observe the form, distribution and emerging time of the mast cells （MCs） and tryptase positive cells （TPCs） in the immune organs of different days old chicken embryos. Methods The thymus, bursa of Fabricius and spleen of chicken embryos at different development time were taken and fixed in Carnoy＇s solution. The alcian blue/safranine O （AB/SO） and streptavidin-biotin complex （SABC） immunohistochemistry were used to observe the MCs and TPCs. Results MCs were first present in the thymus, bursa and spleen of day 13-14 embryos. The number of MCs increased along with the development days. On day 16, TPCs were first found in the immune organs. The form and distribution of TPCs were similar to MCs, but the emerging time of TPCs was later than that of MCs. Conclusion Tryptase appears in the MCs in the immune organs of day 16 or later chicken embryos, and it is mainly located in the MCs of the connective tissues.

关 键 词：鸡胚 类胰蛋白酶阳性细胞 肥大细胞 免疫器官 

分 类 号：R392.12[医药卫生—免疫学] S852.43[医药卫生—基础医学]