骨髓间充质干细胞Fas／FasL介导的免疫调节在结肠炎治疗中的作用  

Role of bone marrow-derived mesenchymal stem cells in treating colitis through Fas /FasL-mediated immune regulation

作　　者：于洋[1,2] 邵秉一[1,2] 帅逸[3] 尚冯青[3] 王茜[4] 周志斐[5] 廖立[3] 金岩[3] 杨德琴[1,2] 

机构地区：[1]重庆医科大学附属口腔医院牙体牙髓科,重庆400015 [2]重庆医科大学附属口腔医院口腔疾病与生物医学重庆市重点实验室,重庆400015 [3]第四军医大学口腔医学院病理教研室,陕西西安710032 [4]第四军医大学口腔医学院第四军医大学附属口腔医院正畸科,陕西西安710032 [5]第四军医大学口腔医学院儿童牙科,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2013年第10期1028-1031,1035,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家重点基础研究发展计划(973)(2011CB964700);重庆市医学重点学科建设经费基金资助项目(20011)

摘　　要：目的探索卵巢摘除术(OVX)所致骨质疏松症小鼠骨髓间充质干细胞(BMSC)与假手术组(sham)BMSC治疗结肠炎效果差异,明确Fas/FasL表达水平改变情况及其下游T淋巴细胞趋化及凋亡程度的差异。方法建立实验性结肠炎模型,OVX小鼠骨质疏松及假手术模型,对比注射OVX组与sham组小鼠BMSC治疗结肠炎的效果,检测2组BMSC对T淋巴细胞趋化及凋亡的差异,采用RT-PCR和Western blot法对BMSC的凋亡相关基因Fas/FasL的表达进行对比。结果注射雌激素缺乏所致骨质疏松小鼠的BMSC与注射假手术组的BMSC相比,表达Fas/FasL降低,从而使T淋巴细胞趋化及凋亡能力减弱,导致用OVX-BMSC治疗结肠炎效果较差。结论 OVX-BMSC通过降低自身Fas/FasL表达减弱肠炎小鼠T淋巴细胞的趋化和凋亡。Objective To investigate the difference in the therapeutic effects of bone marrow-derived mesenchymal stem cells （BMSCs） of mice from ovariectomy （OVX） group and sham group in treating colitis, and then further study the differences of Fas/FasL expression and downstream T cell migration and apoptosis between the two groups. Methods The osteoporosis animal models were set up by ovariectomy in C57BL6 mice. Meanwhile, 3% dextran sulfate sodium （DSS） was administered for inducing colitis. We compared the therapeutic effects of BMSCs from OVX and sham groups in treating colitis, in addition, detected the expression of Fas/FasL in BMSCsby means of RT-PCR and Western blotting. The ability of BMSCs from the two groups of inducing T cell migaration and apoptosis was also detected. Results Compared with the sham group, BMSCs from OVX mice expressed a lower level of Fas/FasL and displayed a decreased ability of inducing T cell migration and apoptosis, thus leading to an inferior therapeutic effect in treating colitis in animal models. Conclusion Fas/FasL expression of BMSCs from the OVX mice is down regulated, thus leading to a decrease of the migration and apoptosis for T cells from mouse colitis.

关 键 词：炎症性肠病 骨髓间充质干细胞 T淋巴细胞 FAS FASL 

分 类 号：R392.12[医药卫生—免疫学] R561.1[医药卫生—基础医学]