葛根芩连汤对2型糖尿病大鼠的治疗作用及其机制探讨  

Therapeutic effects of Gegen Qinlian decoction and its mechanism of action on type 2 diabetic rats

作　　者：李颖萌 范雪梅[2] 王义明[1,2] 梁琼麟[2] 罗国安[1,2] 

机构地区：[1]江西中医学院药学院,江西南昌330004 [2]清华大学化学系,生命有机磷与化学生物学教育部重点实验室,北京100084

出　　处：《药学学报》2013年第9期1415-1421,共7页Acta Pharmaceutica Sinica

基　　金：国家重点基础研究发展计划(973计划)资助项目(2010CB530602);科技部国际合作专项资助项目(2010DFA32420)

摘　　要：为考察葛根芩连汤(GD)抗糖尿病作用及其机制,建立了高脂饲料联合链脲佐菌素(STZ)诱发的大鼠2型糖尿病模型,研究了GD对糖代谢、胰岛素抵抗和氧化应激反应的影响。结果表明,GD可增加模型大鼠肝糖元合成,显著降低空腹血糖和空腹血清胰岛素含量,升高胰岛素敏感指数;增加血清超氧化物歧化酶(SOD)含量,降低丙二醛(MDA)水平;改善胰岛组织和肾组织形态。提示GD可能通过改善糖代谢、改善胰岛素抵抗、提高组织对胰岛素的敏感性和增强机体抗氧化能力,发挥治疗2型糖尿病和保护受损胰岛β细胞的功能。The objective of this study is to fully investigate the therapeutic effect and mechanisms of action of Gegen Qinlian decoction （GD） on type 2 diabetes mellitus （type 2 DM）. A rat model of type 2 DM was established with the combination of high-fat diet and multiple low doses of streptozotocin （STZ）. Biochemical indicators related to glucose metabolism disorders, insulin resistance, oxidative stress were observed. The type 2 DM rats were administrated with GD for 80 days, the above-mentioned indexes were detected. The results indicated that the hepatic glycogen synthesis level was promoted, fasting blood glucose level and fasting blood insulin level were significantly reduced, insulin sensitivity index was significantly improved; the level of superoxide dismutase （SOD） was increased and the level of malondialdehyde （MDA） was reduced; pathologic morphology of pancreas and kidney was ameliorated in the GD group. It was indicated that the therapeutic mechanisms of action of GD on type 2 DM might be related to its effect of ameliorating glucose metabolism disorders, relieving insulin resistance, increasing the tissues＇ sensitivity to insulin, improving the antioxidative ability of living system, GD has therapeutic effect on type 2 DM and protective effects against damaged pancreatic function.

关 键 词：葛根芩连汤 2型糖尿病 糖代谢 胰岛素抵抗 氧化应激 

分 类 号：R965[医药卫生—药理学]