卷柏中穗花杉双黄酮对TNF-α诱导的血管内皮细胞损伤的保护作用  

Protection effect of amentoflavone in Selaginella tamariscina against TNF-α-induced vascular injure of endothelial cells

作　　者：郑晓珂[1] 刘彩霞[1] 翟英英[1] 李玲玲[1] 王小兰[1] 冯卫生[1] 

机构地区：[1]河南中医学院,河南郑州450046

出　　处：《药学学报》2013年第9期1503-1509,共7页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(81073034);国家"重大新药创制"科技重大专项(2013ZX09102-022);国家重点基础研究发展计划(973计划)资助项目(2013CB531802)

摘　　要：卷柏[Selaginella tamariscina（Beauv.）Spring]为卷柏科（Selaginellaceae）植物卷柏的干燥全草,具有活血通经的功效。现代药理及临床研究表明,卷柏提取物具有降血脂、降血糖、抗菌、抗病毒、抗辐射、抗氧化、植物雌激素等作用。本实验室前期在采用去势大鼠模型研究卷柏的雌激素样作用时,意外发现卷柏有良好的降血糖、降血脂作用,This study is to observe the protection effect of amentoflavone （AMT） in Selaginella tamariscina against TNF-α-induced vascular inflammation injury of endothelial cells. On the basis of TNF-α induced human umbilical vein endothelial cell, observe the influence of AMT on endothelial active factor, the contents of SOD and MDA, the protein expression of vascular endothelial adhesion molecules and inflammatory factor; study the effect of its common related signal pathways such as NF- κB; research the effect of AMT against TNF-α induced human umbilical vein endothelial cell injury by means of MTT, ELISA, Western blotting and the cell immunofluorescence. The results showed that AMT could increase the content of NO and decrease the levels of VCAM-1, E-selectin, IL-6, IL-8 and ET-1; enhance the activity of SOD, reduce the content of MDA; down- regulate the protein expressions of VCAM-1, E-selectin, NF-κBp65 and up-regulate IκBα, attenuate the NF- κBp65 transfer to cell nucleus. AMT has the effect of protect vascular endothelial and maybe via the signal pathway of NF-κB to down-regulate the inflammation factor and oxidative damage factor of downstream.

关 键 词：穗花杉双黄酮 人脐静脉内皮细胞 血管内皮损伤 血管内皮保护作用 

分 类 号：R963[医药卫生—微生物与生化药学]