PDGF-BB、TNF-α参与电离辐射致小鼠皮肤创面愈合延迟  

PDGF-BB and TNF-αare involved in delayed skin wound healing in mice exposed to ionizing radiation

作　　者：王国栋[1] 王佳琪[1] 赵云富[1] 柏书博 陈潇卿[1] 吴洋[1] 汪大林[3] 

机构地区：[1]第二军医大学长征医院口腔科,上海200003 [2]解放军85医院口腔科,上海200052 [3]第二军医大学长海医院口腔科,上海200433

出　　处：《第二军医大学学报》2013年第9期954-959,共6页Academic Journal of Second Military Medical University

基　　金：全军医学科技"十二五"科研项目(CWS11J300);上海市科委产学研医合作项目(12DZ1940503);上海市科委医学引导类项目(114119b2300);第二军医大学青年启动基金(2010QN21)~~

摘　　要：目的探讨电离辐射(ionizing radiation,IR)致小鼠皮肤创面愈合延迟可能的细胞因子机制。方法 68只雌性昆明种小鼠随机分为两组(n=34):实验组接受6Gy 60 Coγ射线全身照射后即刻制作背部皮肤缺损;对照组小鼠不接受辐照,同期制作背部皮肤缺损。伤后2、4、6、8、10、12、14d连续描记小鼠(n=10)伤口面积,观察伤口愈合率变化。伤后1、3、5、7d分别处死小鼠(每个时间点各6只小鼠),取创面周围皮肤及下方的薄层肌肉组织,H-E染色评价伤口愈合情况;免疫组化和real-time PCR检测小鼠皮肤缺损组织血小板衍生生长因子BB(PDGF-BB)、肿瘤坏死因子α(TNF-α)的表达水平。结果致伤后14d内各时间点,实验组伤口愈合率均低于对照组(P<0.01);14d时实验组伤口愈合率为61.61%,对照组为90.13%,差异有统计学意义(P<0.01)。H-E染色显示:与对照组相比,实验组伤口内炎性细胞浸润明显,胶原纤维排列无序,成纤维细胞增生减少。免疫组化和real-time PCR结果显示:伤后3～7d实验组PDGF-BB蛋白表达低于对照组,差异有统计学意义(P<0.05);伤后3d,实验组PDGF-BB基因表达低于对照组(P<0.01)。致伤后两组小鼠TNF-α蛋白及基因表达均上调,5d达高峰,其后开始下降;伤后7d时实验组TNF-α蛋白及基因表达均高于对照组(P<0.01)。结论 PDGF-BB、TNF-α参与了IR致小鼠背部皮肤缺损愈合延迟。Objective To explore the possible cytokine mechanism of delayed skin wound healing in mice exposed to ionizing radiation（IR）, so as to provide evidence for further research. Methods Totally 68 female Kunming mice were randomly divided into two groups. Back skin defect was made in mice of the experimental group （n= 34） immediately after exposure to 6 Gy 60Co γ whole-body radiation, and mice in the control group （n=34） had skin defect but with no radiation exposure. Wound areas were continuously measured and wound healing rates were monitored on day 2, 4, 6, 8, 10, 12, and 14 after damage （n=10）. Mice were sacrificed on day 1, 3, 5, and 7 （each time point 6 mice）, the skin and thin layer of muscle tissues around the wound were obtained, and H-E staining was used for evaluating wound healing. Platelet derived growth factor-BB （PDGF-BB） and tumor necrosis factor-alpha （TNF-α） expression levels in the dermal defects were examined by immunohistochemical methods and real-time PCR. Results The wound healing rate in the experimental group was significantlylower than that in the control group within 14 days after damage（P〈0. 01）. On day 14： the wound healing rate in the experimental group was significantly lower than that in the control group （61. 61% and 90. 13%, P〈0.01）. H-Estaining showed more severe inflammatory cell infiltration, disarranged collagen fibers and less proliferation of fibroblasts in the wound of experimental group compared with that of the control group. Immunohistoehemical and real-time PCR results demonstrated that PDGF-BB expression in experimental group was significantly less than that in the control group from day 3 to day 7 （P〈0. 05）. PDGF-BB gene expression in experimental group was less than that in the control group from day 3 （P〈0.01）. The expression of TNF-α protein and gene was up-regulated in both groups and reached the peak on day 5, then began to decrease. On day 7, TNF-α protein and gene expression in the experimental group was si

关 键 词：电离辐射 血小板源性生长因子BB 肿瘤坏死因子α伤口愈合 

分 类 号：R818.895[医药卫生—放射医学]