X连锁凋亡抑制蛋白修饰的脂肪干细胞移植促进心肌梗死后心肌和毛细血管再生的研究  

X-linked inhibitor of apoptotic protein modified adipose-derived stem cells transplantation promotes the regeneration of myocardium and capillaries after myocardial infarction

作　　者：王建宇[1] 耿婕[2] 姜楠[1] 李博[1] 陈晓波[1] 陈庆良[1] 

机构地区：[1]天津市胸科医院心外科,300051 [2]天津市胸科医院心内科,300051

出　　处：《中华老年医学杂志》2013年第9期991-995,共5页Chinese Journal of Geriatrics

基　　金：天津市卫生局科技基金重点项目（10KG120）

摘　　要：目的探索脂肪干细胞（ADSCs）和x连锁凋亡抑制蛋白（XIAP）修饰的ADscs（xIAP—ADSCs）治疗心肌梗死的机制。方法10周的雄性SD大鼠27只，体质量200～300g，数字抽签将大鼠分为心肌梗死对照组（对照组）、ADSCs组、XIAP—ADSCs组各9只。取自体脂肪组织，提取ADSCs行体外培养扩增。应用电转染的方法将xIAP表达质粒导人ADSCs内，进行基因修饰。结扎大鼠冠状动脉左前降支，选取梗死中央及边缘区共5个注射点，按分组每个注射点注射生理盐水、ADSCs悬液或XIAP-ADSCs悬液，未处理组不做任何处理。结果注射4周后，与对照组比较，ADSCs组和XIAP—ADSCs组梗死区面积减小，梗死区中可见少到中量心肌细胞岛和毛细血管。XIAPADSCs组梗死区毛细血管数目（18．65±1．12）个／mm2，较ADSCs移植组（8．68±0．90）个／mm2、对照组（7．64±0．60）个／mm。增多（t值分别为-22．49、29．91，均P〈0．05）。结论自体ADSCs与XIAP—ADSCs可通过增加心肌细胞和毛细血管的生成减小心肌梗死面积，抑制心室重构，从而恢复心功能。Objective To investigate the mechanism of transplantation of autologous adipose- derived stem cells （ADSCs） and X linked inhibitor of apoptosis protein （XIAP） modified ADSCs for the treatment of myocardial infarction （MI） by the histological detection method. Methods Totally 27 male SD rats （aged 10 weeks, weighted 200-300 g） were randomly divided into the control group, ADSCs group and XIAP-ADSCs group （n= 9 each）. ADSCs were isolated from inguinal adipose tissue in rats and were cultivated in Dulbecco＇s modified Eaglets medium. X-linked inhibitor of apoptotic protein expression plasmid was electrotransfected into ADSCs. Myocardial infarction model was conducted by ligating the left anterior descending artery. The control group was not given any operation. Rats in different groups received direct epieardial injections of normal saline, or ADSCs cell suspension or XIAP modified ADSCs cell suspensions respectively at five sites in the central and border zones of myocardial infarction. Results Compared with control group, the infarct areas were decreased in ADSCs and XIAP-ADSCs groups, and some myocardial cells and blood capillaries could be found in the myocardial infarction zone. The capillary number was much more in XIAP-ADSCs group than in ADSCs and control groups 〈（18.65±1.12） /ram2 vs. （8.68±0.90） /mm2 , （18.65± 1.12） /mm2 vs. （7. 64 ± 0. 60）/mm2, t=-22.49, 29.91, both P〈0.05. Conclusions Autologus ADSCs and XIAP ADSCs transplantation may reduce infarction area and inhibit left ventricular remodeling by promoting the regeneration of myocardial cells and blood capillaries, which restores the cardiac function.

关 键 词：干细胞移植 心肌梗死 抗凋亡蛋白类 

分 类 号：R542.22[医药卫生—心血管疾病]