嗅球电刺激对成年大鼠室管膜下区神经发生的影响  

Effect of olfactory bulb stimulation on neurogenesis in subventricular zone of adult rats

作　　者：张广慧[1] 何明利[1] 徐艳[1] 徐丙超[1] 李永明[2] 

机构地区：[1]连云港市第一人民医院神经内科,222002 [2]河南省人民医院神经外科,郑州450000

出　　处：《中华神经医学杂志》2013年第9期875-879,共5页Chinese Journal of Neuromedicine

摘　　要：目的探讨嗅球电刺激对成年大鼠室管膜下区（SVZ）神经前体细胞（NPC）增殖及其向嗅球迁移的影响，并对其相关机制进行初步探讨。方法SD大鼠80只按随机数字表法分为正常对照组、假刺激组、电刺激1d组、电刺激3d组、电刺激1周组、电刺激2周组、电刺激3周组、电刺激4周组，每组10只，后6组大鼠制备嗅球电刺激模型，以5-溴脱氧尿嘧啶（Brdu）标记新生细胞，免疫组化染色观察大鼠SVZ区内源性NPC的增殖，RT—PCR检测嗅球内Prokineticin2（prk2）mRNA的表达；另取大鼠15只，按随机数字表法分为正常对照组、假刺激组和电刺激4周组，每组5只，免疫组化染色观察嗅球内Brdu阳性细胞的表达。结果8组大鼠SVZ区Brdu阳性细胞数不同，差异有统计学意义（F=51．475，P=-0．000）。与正常对照组和假刺激组比较，电刺激1d、3d、1周、2周组大鼠刺激侧Brdu阳性细胞增高，差异有统计学意3L（P〈0．05）；8组大鼠嗅球内prk2mRNA的表达不同，差异有统计学意义（F=154．067，P=-0．000）。与正常对照组和假刺激组比较，电刺激1d、3d、1周、2周、3周、4周组prk2mRNA增加，差异有统计学意义（P〈0．05）；3组大鼠嗅球内Brdu阳性细胞数不同，差异有统计学意义（F36．472，P=-0．000）。电刺激4周组嗅球内Brdu阳性细胞数较正常对照组和假刺激组明显增多，差异有统计学意义（P〈0．05）。结论电刺激嗅球可促进Svz区NPC的增殖并向嗅球迁移，这可能与电刺激提高嗅球内Prk2的表达水平有关。Objective To investigate the effects of electrical stimulation of olfactory bulb （OB） on proliferation, migration to OB of neural precursor cells （NPC） in the subventricular zone, and preliminary explore its related mechanism. Methods Eighty adult female Sprague Dawley rats were randomly divided into normal control group, sham stimulation group and stimulation groups of 1 d, 3 d, 1 week, 2 weeks, 3 weeks and 4 weeks （n=10）. Rats in the later six groups were performed OB electrical stimulation. Five of these rats in each group were injected intraperitoneally with 5-bromo-2-deoxyuridine （Brdu） to mark new-born cells and immunohistochemical staining was employed to observe the proliferation of NPCs in SVZ; the other 5 were sacrificed at corresponding time to detect the mRNA expression ofprokineticin 2 （prk2） in OB. Another 15 rats were randomly divided into normal control group I, sham stimulation group I and stimulation group I （n=5）; four weeks after Brdu was injected, the animals were sacrificed and immumohistochemical staining was used to investigate the number of Brdu-positive cells in OB. Results The number of Brdu-positive cells in SVZ was significantly different among the eight group （F=51.475, P=0.000）; as compared with those in the normal control group and sham stimulation group, the Brdu-positive cells were significantly increased in stimulation groups of 1 d, 3 d, 1 week and 2 weeks （P〈0.05）. The mRNA expression ofprk 2 in OB was significantly different among the eight group （F=154.067, P=0.000）; as compared with those in the normal control group and sham stimulation group, the Brdu-positive cells were significantly increased in all the stimulation groups （F=36.472, P=0.000）. Four weeks after injection of Brdu, the Brdu-positive cells in OB of the stimulation group I significantly increased as compared with that in the normal control group I and sham stimulation group I （P〈0.05）. Conclusion Electrical stimulation of OB promotes proliferation, migration of N

关 键 词：电刺激 嗅球 室管膜下区 神经前体细胞 Prokineticin 2 

分 类 号：R338[医药卫生—人体生理学]