临床分离副溶血弧菌药敏表型和毒力特征分析  

Antimicrobial resistance and virulence characteristics of Vibrio parahaemolyticus

作　　者：孙宇峰[1] 范艳艳[1] 黄艳飞[1] 王玫[1] 鲁辛辛[1] 

机构地区：[1]首都医科大学附属北京同仁医院检验科,100730

出　　处：《中华医学杂志》2013年第34期2733-2735,共3页National Medical Journal of China

摘　　要：目的了解临床分离的副溶血弧菌的耐药性及毒力特征。方法通过医生工作站获取患者临床资料并通过实验室信息管理系统（LIS）统计2009至2012年北京同仁医院肠道门诊细菌性腹泻病原菌种类及数量；纸片扩散法检测副溶血弧菌对15种抗生素的耐药表型；PCR技术检测直接溶血毒素（TDH）基因（tdh）、TDH-相关溶血毒素基因（trh）和两个Ⅲ型分泌系统2基因（T3SS2a、T3S8213）。结果副溶血弧菌在细菌性腹泻中所占比例为37．90％（274／723），居于首位。216株副溶血弧菌对氨苄西林、头孢噻吩、头孢呋辛、阿米卡星4种抗生素的耐药率分别为100％（216／216）、44．91％（97／216）、24．07％（52／216）、9．26％（20／216）。对另外11种抗生素敏感或中度敏感。216株副溶血弧菌中tdh携带率为96．76％（209／216），trh携带率为1．39％（3／216）；T3SS2携带率为100％，其中213株只携带T3SS2a，2株只携带T3SS213，1株同时携带T3SS2a和T3SS213。结论副溶血弧菌对氨苄西林耐药率最高，对其他抗生素不同程度耐药。造成人类腹泻的副溶血弧菌主要是tdh阳性菌株；Ⅲ型分泌系统在菌株致病中起重要作用。Objective To explore drug resistance and virulence characteristics of clinically isolated Vibrio parahaernolyticus （VP）. Methods The patient records were reviewed. The laboratory information system was employed to count the quantity of bacterial diarrhea pathogens at our intestinal clinic from 2009 to 2012. The drug resistant phenotype was tested by the method of disk diffusion. Polymerase chain reaction （PCR） was used to detect thermo-stable direct hemolysin （TDH） gene （tdh） , TDH-related hemolysin gene （trh） and type Ⅲ secretion system 2 （T3 SS2a, T3SS2a ）. Results The VP infection rate was 37.90% （274/723）. Susceptibility test showed that the resistance rates of ampicillin, cefalotin, cefuroxime and amikacin were 100% （216/216）, 44. 91% （97/216） ,24. 07% （52/216） ,9. 26% （20/216）. The other 11 kinds of antibiotics were sensitive or intermediate. 96. 76% （209/216） VP harbored tdh gene, 1.39% （3/216） trh gene and 100% T3SS2 gene, with 213 strains T3SS2a positive, 2 strains T3SS2a positive and 1 strain both T3SS2a and T3SS213 positive. Conclusion The resistant rate of VP to ampicillin is the highest and VP has different levels of resistance against other antibiotics. Most clinical isolates of VP harbor tdh gene and T3SS2 plays an important role in the pathogenicity of VP.

关 键 词：弧菌 副溶血性 抗药性 细菌 溶血毒素 Ⅲ型分泌系统 

分 类 号：R446.5[医药卫生—诊断学]