红藤方对内异症MMP-9表达的影响  

Effect of Caulis Sargentodoxae Formula on MMP-9 expression in endometriosis

作　　者：戚潜辉[1] 陈美苑[1] 钟成[1] 范杰平[1] 陈盛强[1] 

机构地区：[1]广州医科大学第二附属医院,广东广州510260

出　　处：《解剖学研究》2013年第4期273-275,319,共4页Anatomy Research

基　　金：广东省中医药局科研课题(2010218)

摘　　要：目的探讨红藤方对子宫内膜异位症小鼠MMP-9表达的影响。方法子宫内膜异位小鼠模型,随机分为模型组和实验组,实验组口服给药红藤方。21 d后,分别取出子宫异位内膜组织,观察组织形态学变化及采用免疫组织化学方法检测基质金属蛋白酶9(MMP-9)的表达。结果在光学显微镜下观察,发现用药后异位组织周围血管减少及异位内膜腺体萎缩,腺细胞核固缩,MMP-9表达减少。结论红藤方能抑制异位内膜增生,其治疗机制可能与抑制MMP-9的表达有关系。Objective To explore the effect of Caulis Sargentodoxae Formula on expression of MMP-9 in the ectopic en- dometrium model mice. Methods The model mice were divided randomly into 2 groups： model group and experimental group. Ex- perimental group were treated by Caulis Sargentodoxae Formula. After 21 days continuous treatment ectopic endometrial tissue were removed and histomorphology changes and matrix metalloproteinase-9 ~MMP-9） expression were examined by hematoxylin-eosin （HE） staining and immunohistochemistry method respectively. Results Less peripheral vascellum and ectopic endometrium and pycnosis of glandular cell nucleus are found by optical microscope observation in experimental group tissue. Expression of MMP-9 are reduced also. Conclusion Caulis Sargentodoxae Formula can inhibit the growth of the ectopic endometrium. The decrease ex- pression of MMP-9 in ectopic endometrium may be relevant to its the treatment mechanism.

关 键 词：子宫内膜异位症 红藤方 基质金属蛋白酶9 小鼠 

分 类 号：R285[医药卫生—中药学]