BDNF对大鼠急性脊髓损伤后NF-κB表达的影响  

Effect of BDNF on the expression of NF-κB in acute spinal cord injury rat

作　　者：王岩峰[1] 秦光华[1] 张玉强[1] 杨明超[1] 王喆[1] 

机构地区：[1]中国医科大学附属第一医院骨科,辽宁沈阳110001

出　　处：《解剖学研究》2013年第4期283-286,共4页Anatomy Research

基　　金：国家自然科学(81100924);教育部高校博士学科点专项科研基金(20092104120006);辽宁省教育厅科研基金(L2010687)

摘　　要：目的研究脑源性神经营养因子(BDNF)对急性脊髓损伤大鼠核转录因子-κB(nuclear factor-kappa B,NF-κB)表达的变化。方法参照Nystrom压迫方法制作大鼠脊髓压迫损伤模型,成年健康Wistar大鼠72只,雌雄不限,按随机数字表法分为正常对照组8只、损伤组32只、BDNF组32只。免疫组化和Western blot检测各组大鼠NF-κB的表达。结果免疫组化结果显示:损伤组与BDNF组NF-κB阳性产物的平均光密度值(mean optic density,MOD)显著高于正常对照组(P<0.01);而BDNF组与损伤组相比A值明显降低(P<0.01);Western blot结果显示:与正常对照组比较,损伤组与BDNF组NF-κB的灰度值(integrated density value,IDV)与内参照IDV的比值明显升高(P<0.01),而BDNF组则明显低于损伤组(P<0.01)。结论 BDNF很可通过下调NF-κB的表达参与脊髓继发性损伤。Objective To study the expression of nuclear factor-kappa B （NF-κB） in acute spinal cord injury rat after BDNF was injected. Methods The spinal cord injury was induced with Nystrom way. The healthy adult Wistar rats （72） were randomly divided into normal control group, spinal cord injury group, BDNF group on average. The expression of NF-κB was observed by immunochemistry and western blot methods in all groups. Results The mean optic density （A） of NF-κB positive product increased significantly in acute spinal cord injury group and BDNF group than in normal control by means of immunochemistry （P〈O.01）; but decreased in the BDNF group than in the acute spinal cord injury group （P〈0.01）. The ratios between integrated density value （IDV） for NF-κB protein band and IDV for 13-actin protein band increased significantly in the spinal cord injury group and BDNF group than in normal control by means of Western blot （P〈0.01） ; but decreased in the BDNF group than in the spinal cord injury group （P〈0.01）. Conclusion One of the mechanisms for BDNF participate the pathogenesis of acute spinal cord injury might be upregulate the expression of NF-κB.

关 键 词：脊髓损伤 脑源性神经营养因子 核转录因子-ΚB 

分 类 号：R651.2[医药卫生—外科学]