Heme oxygenase-1 promotes Caco-2 cell proliferation and migration by targeting CTNND1  

作　　者：ZHANG Li LIU Yu-lin CHEN Guang-xiang CUI Bin WANG Jin-shen SHI Yu-long LI Le-ping GUO Xiao-bo 

机构地区：[1]Department of Gastrointestinal Surgery, Provincial Hospital Affiliatedto Shandong University, Jinan, Shandong 250021, China

出　　处：《Chinese Medical Journal》2013年第16期3057-3063,共7页中华医学杂志（英文版）

基　　金：This study was supported by grants from the Key Research Project from Shandong Science and Technology Commission （No. 2011GSF 11846） and the National Youthful Science Foundation of China （No. 81101858）.

摘　　要：Background Heme oxygenase-1 （HO-1） can be induced by inflammatory cytokines,oxidation,ischemia,hypoxia,and endotoxins.As a ＂graft survival protective gene,＂ HO-1 is a hot spot in organ transplantation research.However,the role of HO-1 gene expression in the function of human colon adenocarcinoma cell line （Caco-2） cells has not been reported previously.Methods The role of HO-1 in the proliferation and migration of Caco-2 cells was analyzed using a stable HO-1 expression plasmid.We constructed a recombinant adeno-associated virus plasmid containing the HO-1 gene,heme oxygenase 1 （HMOX1）,which was transfected into Caco-2 intestinal cells.We identified a number of target genes by global microarray analysis combined with real-time polymerase chain reaction （PCR） and chromatin immunoprecipitation assay.Results Our results showed that significant HO-1 upregulation was demonstrated in the Caco-2 cells after HO-1 transfection.Restoration of HO-1 expression promoted proliferation and invasion in vitro.The CTNND1 gene,a member of the armadillo protein family,was identified as a direct HO-1 target gene.Conclusion Overexpression of HO-1 promotes Caco-2 cell proliferation and migration by targeting the CTNND1 gene.Background Heme oxygenase-1 （HO-1） can be induced by inflammatory cytokines,oxidation,ischemia,hypoxia,and endotoxins.As a ＂graft survival protective gene,＂ HO-1 is a hot spot in organ transplantation research.However,the role of HO-1 gene expression in the function of human colon adenocarcinoma cell line （Caco-2） cells has not been reported previously.Methods The role of HO-1 in the proliferation and migration of Caco-2 cells was analyzed using a stable HO-1 expression plasmid.We constructed a recombinant adeno-associated virus plasmid containing the HO-1 gene,heme oxygenase 1 （HMOX1）,which was transfected into Caco-2 intestinal cells.We identified a number of target genes by global microarray analysis combined with real-time polymerase chain reaction （PCR） and chromatin immunoprecipitation assay.Results Our results showed that significant HO-1 upregulation was demonstrated in the Caco-2 cells after HO-1 transfection.Restoration of HO-1 expression promoted proliferation and invasion in vitro.The CTNND1 gene,a member of the armadillo protein family,was identified as a direct HO-1 target gene.Conclusion Overexpression of HO-1 promotes Caco-2 cell proliferation and migration by targeting the CTNND1 gene.

关 键 词：heme oxygenase-1 Caco-2 cells MICROARRAY CTNND1 

分 类 号：Q241[生物学—细胞生物学] Q517