巴戟天离体培养及植株再生的研究  

In-Vitro Culture and Plant Regeneration of Morinda officinalis How

作　　者：贺红[1] 肖省娥[1] 冼建春[1] 徐鸿华[1] 

机构地区：[1]广州中医药大学中药学院,广州510405

出　　处：《广州中医药大学学报》2000年第4期353-354,共2页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省自然科学基金博士启动基金资助! (编984117);国家自然科学基金!(编号 :30 0 70 92 4)

摘　　要：以巴戟天无菌苗的茎尖、带节茎、无节茎为材料进行离体培养。结果表明 :外植体表面消毒采用70 %乙醇预处理 30s ,再用 0 .1%的升汞浸泡 10～ 15min ,效果较好。茎尖和带节茎培养 ,于含有 6-苄基氨基嘌呤 ( 6-benzylaminopurine。BA)的MurashigeandTucker (MT)培养基上 ,均能直接出芽 ,带节茎出芽率最高 ,为 97.5%。适宜的BA浓度为 1mg·L- 1,BA浓度升高 ,出芽率下降。根的诱导 ,以MT加 0 .2～0 .5mg·L- 1的萘乙酸 (naphthleneaceticacid ,NAA)培养基较好 ,生根率可达 80 .0The sterile stem tips, nodular and non-nodular stem of Morinda officinalis How were cultured in vitro. The results showed that the most suitable procedure for sterilization of explants was soaking into 0.1% HgCl 2 solution for 10 to 15 minutes after pretreating with 70% ethanol for 30 seconds. MT culture medium with BA was effective to induce direct shooting of stem tips and nodular stem, the shooting rate of nodular stem being 97.5% . The optimal BA concentration was 1 mg·L -1 , the shooting rate would decrease when the concentration of BA increased. As for the induction of rooting, MT culture medium adding with 0.2 to 0.5 mg·L -1 NAA was optimal, the rooting rate being over 80.0% .

关 键 词：巴戟天 生长 发育 离体培养 植株再生 

分 类 号：S567[农业科学—中草药栽培]