细胞因子抗体及地塞米松对小鼠脓毒血症心肌及骨骼肌ATP含量的影响  

Influences of Antibody of Cytokine and Dexamethasone on the Content of ATP in Myocardium and Skeletal Muscle of Sepsis Mice

作　　者：欧阳彬[1] 王吉甫[1] 吴珏恒[2] 陈敏英[1] 刘仁斌[1] 张清秀[3] 

机构地区：[1]中山医科大学附属第一医院外科,广东广州510080 [2]中山医科大学分子医学中心,广东广州510089 [3]中山医科大学生物化学教研室,广东广州510089

出　　处：《中山医科大学学报》2000年第6期442-444,447,共4页Academic Journal of Sun Yat-sen University of Medical Sciences

基　　金：中山医科大学青年科研基金资助项目(9824)

摘　　要：【目的】观察和比较细胞因子抗体及地塞米松 (DXM )对脓毒血症心肌及骨骼肌细胞腺苷三磷酸 (ATP)含量的影响。【方法】制作小鼠盲肠结扎穿孔 (CLP)脓毒血症模型 ,分别注射生理盐水、IL 1βAb、TNFαAb、IFNγAb、IL 1βAb +TNFαAb+IFNγAb ,以及DXM ,观察各组小鼠心肌及骨骼肌ATP含量。【结果】①CLP模型心肌及骨骼肌细胞ATP含量严重减少。②IL 1βAb、TNFαAb使CLP模型小鼠心肌和或骨骼肌ATP含量轻度增加。而IL 1βAb +TNFαAb +IFNγAb ,以及DXM使CLP模型小鼠心肌及骨骼肌ATP含量增加较为明显 ,其中DXM使CLP模型心肌、骨骼肌细胞ATP含量增加 6～ 8倍。【结论】IFNγAb、DXM能显著改善脓毒血症小鼠心肌、骨骼肌细胞ATP储存。Objective To observe the influences of antibody of cytokine and dexamethasone (DXM) on the content of ATP of myocardium in sepsis model. Methods 9 g/L NaCl, IL-1 βAb, TNF αAb, IFN γAb, IL-1 βAb+TNF αAb+IFN γAb, and DXM was injected into sepsis model of cecum ligation puncture (CLP) respectively. The content of ATP in myocardium and skeletal muscle was observed in each group. Results ①The content of ATP in myocardium and skeletal muscle was very low in the CLP group. ②IL-1 βAb and TNF αAb increased the ATP content of myocardium and /or skeletal muscle in CLP mice in some content. IFN γAb, IL-1 βAb+TNF αAb+IFN γAb and DXM made the ATP content increased more obviously. DXM made the ATP content increased 6～8 times compared with that of CLP group. Conclusions DXM and IFN γAb increased the content of ATP of myocardium and skeletal muscle obviously in sepsis mice.

关 键 词：脓毒症 地塞米松/药理学 疾病模型 动物 腺苷三磷酸 抗体 白细胞介素 抗体 肿瘤坏死因子 抗体 干扰素 

分 类 号：R631.2[医药卫生—外科学]