气相色谱外标法测定生物柴油中的酯类组分  

Determination of ester compounds in biodiesel using gas chromatography with external standard method

作　　者：张琪[1] 张继龙[1,2] 王会刚[1] 王鹏[1] 马静红[1] 李瑞丰[1] 

机构地区：[1]太原理工大学精细化工研究所,山西太原030024 [2]太原理工大学矿业工程学院,山西太原030024

出　　处：《现代化工》2013年第8期134-138,共5页Modern Chemical Industry

基　　金：国家自然科学基金(51172154);山西省回国留学人员重点项目(2011-重点2);太原市重大专项(110147)

摘　　要：建立了测定生物柴油样品中脂肪酸甲酯、单脂肪酸甘油酯、二脂肪酸甘油酯和三脂肪酸甘油酯的气相色谱(外标法)检测方法。使用吡啶作溶剂,采用热柱头进样和DB-5ht毛细柱分离,全自动进样器程序控制进行氢火焰离子检测。检测得出最佳进样质量浓度为1.0～1.5 mg/mL,所选进样浓度范围内各样品回收率≥99.7%,线性良好(r=0.996 7～0.999 8)。并测定了生物柴油主要组分的标样保留时间和线性回归方程,且本气相色谱分析方法具有非常好的重复性和高精确度,各组分相对标准偏差范围在0.13%～1.66%,在实际的生物柴油样品检测分析中体现了相当高的检测准确性。A method for determination of compounds in biodiesel such as fatty acid methyl ester （FAME）,monoglyceride,diglyceride and triglyceride,is established using gas chromatography with external standard method.The sample is dissolved in pyridine and injected by automatic injection.Gas chromatography analysis is performed on a Agilent-DB-5ht capillary column couple with flame ionization detector （FID）.The limitation of detection is 0.05-1.5 mg/mL with linearity correlation coefficient of 0.996 7-0.999 8.And the optimum sample concentration is 1.0-1.5 mg/mL.The detection recoveries of standard compounds of biodiesel are more than 99.7％ with the relative standard deviation （n =5） of 0.13％-1.66％,which show the high accuracy of the detection method.This method can be routinely for the determination of four kinds of ester compounds in biodiesel products.

关 键 词：气相色谱 外标法 自动进样 生物柴油 甘油三酯 

分 类 号：O658[理学—分析化学]