上运动神经元损伤后骨骼肌AChR的改变及CGRP的调控作用  

Changes of acetylcholine receptor in skeletal muscle after upper motoneuron injury and the regulation of calcitonin gene related peptide

作　　者：王庚[1] 吴新民[1] 

机构地区：[1]北京医科大学第一医院麻醉科,100034

出　　处：《中华麻醉学杂志》2000年第11期681-683,共3页Chinese Journal of Anesthesiology

摘　　要：目的　观察大鼠上运动神经元损伤后 ,骨骼肌乙酰胆碱受体γ亚单位和ε亚单位mRNA的变化以及脊髓前角降钙素基因相关肽的变化。方法　选用成年雄性Wistar大鼠 ,戊巴比妥钠麻醉下于下胸段行脊髓横断术 ,术后分别于 0、1、2、4、7和 14d取材横断水平以下的脊髓和下肢腓肠肌。应用Northern杂交方法测定肌肉中乙酰胆碱受体γ亚单位和ε亚单位mRNA的含量 ,应用放射性免疫方法测定脊髓前角降钙素基因相关肽的含量。结果　肌肉中乙酰胆碱受体γ亚单位mRNA自术后第 4d起与正常值比较显著性提高 ,14d时达到最高值 ;而ε亚单位mRNA直至术后 14d才与正常值的差异有统计学意义。脊髓前角降钙素基因相关肽的含量术后即减少 ,自术后第 2d起降至正常水平的 5 0 %～ 80 %。结论　上运动神经无损伤后 ,肌肉中乙酰胆碱受体γ和ε亚单位mRNA的含量显著增加 ,降钙素基因相关肽对γ和ε亚单位的表达可能有调控作用。Objective To observe the time profile of the changes of mRNA of γ and ε subunit of acetylcholine receptor (AChR) in skeletal muscle and the level of calcitonin gene related peptide (CGRP) in spinal motoneuron after upper motoneuron injury. Method The spinal cord of adult male Wistar rats was transected on the lower thoracic segment. The spinal cords below the transection level and soleus muscles were sampled 0,1,2,4,7 and 14 days after surgery, respectively, to detect the levels of mRNA of γ and ε subunit of acetylcholine receptor in soleus muscle using Northern blot method,and CGRP level in spinal motoneuron using radioimmunoassay.Results The levels of mRNA of γ and ε subunit of acetylcholine receptor siginificantly increased after surgery, but the level of mRNA of γ subunit increased earlier and higher than ε subunit. The level of CGRP in spinal motoneuron markedly reduced after surgery, was maintained at 50 80% of normal level since the 2nd day after surgery. Conclusions The levels of AChR γ and ε subunit in skeletal muscle significantly increase after upper motoneuron injury, the expression of which CGRP may regulate.

关 键 词：骨骼肌 ACHR 上运动神经元损伤 调控作用 CGRP 

分 类 号：R971.8[医药卫生—药品]