Using optical tweezers to investigate the specific single-interaction between apoA-I molecule and ABCA1 on living cells  

作　　者：余杰 佟训靓 李成彬 黄一宁 叶安培 

机构地区：[1]Key Laboratory for the Physics and Chemistry of Nanodevices,School of Electronics Engineering and Computer Science,Peking University [2]Department of Neurology,Peking University First Hospital

出　　处：《Chinese Optics Letters》2013年第9期76-79,共4页中国光学快报（英文版）

基　　金：supported by the National Basic Research Program of China(No.2011CB809100);the National Science and Technology Infrastructure Program of China(No.2012BAF14B14);the National Key Technologies R&D Program of China(No.2012ZX09303005003)

摘　　要：We carry out in situ single-molecule measurements of the specific interaction between apolipoprotein A-I （apoA-I） and ATP binding cassette transporter A1 （ABCA1） on THP-1 cells. Single-molecule force spectroscopy shows that similar to normal apoA-I, the dysfunctional apoA-I from diabetes patients interacts with ABCA1 via two different binding sites on the cells. The strength of dysfunctional apoA-I binding to a high-capacity binding site is 26.5±4.9 pN. The minor direct apoA-I/ABCA1 binding strength is 56.7±4.1 pN. These results facilitate a pathological understanding of the mechanisms that underlie the specific interaction of aDoA-I and ABCA1 at the single-molecule level.We carry out in situ single-molecule measurements of the specific interaction between apolipoprotein A-I （apoA-I） and ATP binding cassette transporter A1 （ABCA1） on THP-1 cells. Single-molecule force spectroscopy shows that similar to normal apoA-I, the dysfunctional apoA-I from diabetes patients interacts with ABCA1 via two different binding sites on the cells. The strength of dysfunctional apoA-I binding to a high-capacity binding site is 26.5±4.9 pN. The minor direct apoA-I/ABCA1 binding strength is 56.7±4.1 pN. These results facilitate a pathological understanding of the mechanisms that underlie the specific interaction of aDoA-I and ABCA1 at the single-molecule level.

关 键 词：Binding sites Optical tweezers 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]