SphK1信号通路对高糖诱导的系膜细胞FN表达的影响  

The effect of SphK1 signaling on high glucose-induced fibronectin expression in mesangial cells

作　　者：兰天[1] 黄凯鹏[2] 吴腾[1] 勾红菊[1] 王丽京[1] 黄河清[2] 

机构地区：[1]广东药学院血管生物学研究所 [2]中山大学药学院,广东广州510006

出　　处：《广东药学院学报》2013年第3期318-321,共4页Academic Journal of Guangdong College of Pharmacy

基　　金：国家自然科学基金(81170676;81200308;31271455);广东省自然科学基金(S2012020010991);广东企业科技特派员工作站基金(2011B090600033)

摘　　要：目的研究阻断鞘氨醇激酶1(sphingosine kinase 1,SphK1)通路对高糖诱导的大鼠系膜细胞(glomerular mesangial cells,GMC)中纤维连接蛋白(fibronectin,FN)表达的抑制作用。方法采用RealtimePCR检测SphK mRNA水平;Western blot检测FN的蛋白表达。结果 SphK1抑制剂二甲基鞘氨醇(N,N-Dimethylsphingosine,DMS)显著降低高糖诱导的FN表达;转染SphK1 siRNA可有效消除高糖诱导的FN上调效应。结论 SphK1通路在高糖诱导的肾小球系膜细胞FN表达过程中发挥着重要的调节作用。Objective To investigate the effect of sphingosine kinase 1 （SphK1） signaling on high glucose- induced fibronectin （FN） expression in glomerular mesangial cells （GMC）, Methods The mRNA level of SphK was measured by real-time PCR. The expression of FN was examined by western blot. Results Treatment with N,N-Dimethylsphingosine （DMS） significantly inhibited FN expression induced by high glucose. Transfection of SphKl siRNA abolished FN expression induced by high glucose. Conclusion SphK1 signaling plays a critical role in modulation the expression of FN in high ~[ueose-induced GMC.

关 键 词：SphK1通路 纤维连接蛋白 糖尿病肾病 系膜细胞 

分 类 号：R587.2[医药卫生—内分泌]