肺结核病人T淋巴细胞亚群的变化研究  

Study on Changes of T-lymphocyte Subsets in Patients with Pulmonary Tuberculosis

作　　者：张健[1] 王宝英[1] 王昕升[1] 武云慧[1] 宋爱华[1] 

机构地区：[1]秦皇岛市第三医院检验科,河北秦皇岛066000

出　　处：《现代检验医学杂志》2013年第4期104-105,107,共3页Journal of Modern Laboratory Medicine

基　　金：基金项目：秦皇岛市科技支撑计划项目（编号：201101A237）.

摘　　要：目的 探析肺结核患者T淋巴细胞亚群变化的临床意义.方法 选取秦皇岛市第三医院2009年6月～2012年6月住院的初治肺结核患者63例,应用流式细胞术（flow cytometry,FCM）测定肺结核患者外周血CD3＋,CD4＋,CD8＋ T淋巴细胞绝对计数和CD4＋/CD8＋比值,观察肺结核组和健康对照组之间,肺结核病人各年龄组之间的差异;并追踪化疗方案2HRZS（E）/4HR治疗3个月后的数据变化.结果 肺结核病人CD3＋,CD4＋T淋巴细胞明显下降,CD4%明显上升,差异有统计学意义（t=-3.335~2.655,P=0.002~0.012）.治疗3个月后肺结核病人CD3＋,CD4＋,CD4＋/CD8＋绝对值计数明显升高（t=2.085~2.605,P=0.01~ 0.039）.结论 T淋巴细胞亚群可作为判断肺结核患者免疫功能的指标,这对于了解肺结核患者的免疫状态及指导治疗有一定意义.Objective To explore the changes of T-lymphocyte subsets in patients with pulmonary tuberculosis and its clinical significance. Methods 63 cases primary pulmonary tuberculosis during June 2009 to June 2012 in Qinhuangdao City the Third Hospital were selected and detected by Flow Cytometry（FCM） for venous blood CD3＋ ,CD4＋ , CD8＋ T-lymphocyte absolute counts and CD4＋/CD8＋ ratio,with 28 healthy persons as control group. The difference among types of pulmonary tuberculosis,age groups were analyzed,and track chemotherapy 2HRZS（E）/4HR changes of data after three months treat- ment. Results The CD3＋ ,CD4＋ T-lymphocyte absolute counts dropped,and CD4＋ increased ascompared with the control group （t= -- 3. 335 ~ 2. 655, P = 0. 002 ~ 0. 012）. Treatment of pulmonary tuberculosis patients after 3 months count of CD3＋ ,CD4＋ ,CD4＋/CD8＋ theabsolute value markedly increased （t = 2. 085 ~ 2. 605, P = 0. 01 ~ 0. 039）. Conclusion T- lymphocyte subsets can be used as indication of the immune function in pulmonary tuberculosis patients. It is clinical signifi- cance with regard to the understanding of the immune status, the treatment effect of pulmonary tuberculosis patients.

关 键 词：肺结核 T淋巴细胞亚群 流式细胞术 

分 类 号：R521[医药卫生—内科学] R392.11[医药卫生—临床医学]