不对称熔解曲线PCR法和DNA测序法检测HBV A1762T/G1764A结果分析  

Asymmetric melting curve PCR method and DNA sequencing method detecting HBV A1762T/G1764A results analysis

作　　者：刘京伟[1] 赵安成[1] 

机构地区：[1]山东省临沂市沂水中心医院,山东临沂276400

出　　处：《医学检验与临床》2013年第4期10-11,共2页Medical Laboratory Science and Clinics

摘　　要：目的：以DNA测序法为标准，探讨不对称PCR熔解曲线法检测乙型肝炎病毒A1762T/G1764A基因变异的敏感性和特异性。方法：选取45例HBV阳性且经PCR熔解曲线法显示A1762T/G1764A变异的乙型肝炎患者血清，另选10例未发生HBV A1762T/G1764A变异的慢乙肝患者血清，用DNA测序法进行比较，分析两者检测结果。结果：55例测序样本中有51例两法结果完全一致,总符合率92.7%，另4例结果是：不对称PCR熔解曲线法检测显示为不完全变异型,而测序法则显示为完全变异型。结论：两法的相符率高，PCR熔解曲线法对A1762T/G1764A变异的检测是一简单易行较实用的方法，适用于临床基层开展。Objective：Taking DNA sequencing methods as the standard, to explore the sensitivity and and specificity of A1762T/G1764A mutation by PCR melting curve method. Methods：Choose 45 samples that the serumA1762T/G1764A mutation of patients were detected by PCR melting curve method, there patients HBV-DNA. Moreover choose other 10 samples that have not had the A1762T/G1764A variation. All detected by DNA sequencing method. Analysis results in different methods.Results：In the 55 samples, there are 51 samples whose result sare consistent, the total coincidence rate is 92.7%.Conclusions：The coincidence rate of two methods is high. It means that using PCR melting curve method to detect A1762T/G1764A variation is an easy and feasible practical method, and is suitable used in clinical and the basic units.

关 键 词：溶解曲线PCR A1762T G1764A DNA序列 

分 类 号：R512.604[医药卫生—内科学]