Novel crosslinked alginate/hyaluronic acid hydrogels for nerve tissue engineering  

作　　者：Min-Dan WANG Peng ZHAI David J. SCHREYER Ruo-Shi ZHENG Xiao-Dan SUN Fu-Zhai CUI Xiong-Biao CHEN 

机构地区：[1]Division of Biomedical Engineering, University of Saskatchewan, 57 Campus Dr., Saskatoon, SK S7N5A9, Canada [2]Department of Anatomy and Cell Biology, University of Saskatchewan, 107 Wiggins Rd., Saskatoon, SK S7N5E5, Canada [3]School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China [4]Department of Mechanical Engineering, University of Saskatchewan, 57 Campus Dr., Saskatoon, SK S7N5A9, Canada

出　　处：《Frontiers of Materials Science》2013年第3期269-284,共16页材料学前沿（英文版）

摘　　要：CT： Artificial tissue engineering scaffods can potentially provide supportand guidance for the regrowth of severed axons following nerve injury. In this study a hybrid biomaterial composed of alginate and hyaluronic acid （HA） was synthesized characterized in terms of its suitability for covalent modification, biocompatibility fir living Schwann cells and feasibility to construct three dimensional （3D） Carbodiimide mediated amide formation for the purpose of covalent crosslinking of the HA was carried out in the presence of calcium ions that ionically crosslink alginate.Amide formation was found to be dependent on the concentrations of cabodiimide and calclum chloride. The double-crosslinked composite hydrogels display blocompatibllity that is comparable to simple HA hydrogels, allowing for Schwann cell survival and significant difference was found between composite hydrogels made from different of alginate and HA. A 3D BioPIotterTM rapid prototyping system was used to fabricats 3D scaffolds. The result indicated that combining HA with alginate facilitated the fabrication process and that 3D scaffolds with porous inner structure can be fabricated ;from the composite hydrogels, but not from HA alone. This information provides a basis for continuing in vitro and in vivo tests of the suitability of alginate/HA hydrogel as a biomaterial to create living cell scaffolds to support nerve regeneration.CT： Artificial tissue engineering scaffods can potentially provide supportand guidance for the regrowth of severed axons following nerve injury. In this study a hybrid biomaterial composed of alginate and hyaluronic acid （HA） was synthesized characterized in terms of its suitability for covalent modification, biocompatibility fir living Schwann cells and feasibility to construct three dimensional （3D） Carbodiimide mediated amide formation for the purpose of covalent crosslinking of the HA was carried out in the presence of calcium ions that ionically crosslink alginate.Amide formation was found to be dependent on the concentrations of cabodiimide and calclum chloride. The double-crosslinked composite hydrogels display blocompatibllity that is comparable to simple HA hydrogels, allowing for Schwann cell survival and significant difference was found between composite hydrogels made from different of alginate and HA. A 3D BioPIotterTM rapid prototyping system was used to fabricats 3D scaffolds. The result indicated that combining HA with alginate facilitated the fabrication process and that 3D scaffolds with porous inner structure can be fabricated ;from the composite hydrogels, but not from HA alone. This information provides a basis for continuing in vitro and in vivo tests of the suitability of alginate/HA hydrogel as a biomaterial to create living cell scaffolds to support nerve regeneration.

关 键 词：hyaluronic acid （HA） ALGINATE hydrogel scaffold nerve injury TISSUEENGINEERING 

分 类 号：TQ317.3[化学工程—高聚物工业] TQ334.9