免疫组织化学HE染片退色方法研究  

Study on the method of decoloration for immunohistochemical HE slides

作　　者：王兴波[1] 王静 万晓书[2] 王东梅[3] 任仕俊[1] 

机构地区：[1]秦皇岛市第二医院病理科,河北省秦皇岛066600 [2]秦皇岛市第二医院肿瘤科,河北省秦皇岛066600 [3]秦皇岛市第二医院内科,河北省秦皇岛066600 [4]昌黎县妇幼保健院病理科

出　　处：《中国基层医药》2013年第22期3420-3422,共3页Chinese Journal of Primary Medicine and Pharmacy

摘　　要：目的探讨HE染片改做免疫组织化学染色时采用不同退色方法对组织抗原表达的影响。方法利用免疫组织化学过程中的抗原修复液（EDTA或柠檬酸溶液）对HE染片进行抗原热修复退色，并与盐酸溶液常温退色法进行比较。结果抗原热修复退色完全彻底，与其它方法相比，退色时间短（1～2min），完全退色率均为90％以上，差异均有统计学意义（x^2=27．6、23．9，均P〈0．01），且不损伤染片，免疫组织化学结果准确，无背景着色和异常着色，抗原表达率及强度高。结论抗原热修复退色不但简化了染色程序，而且能较好地保护组织抗原活性，是目前较好的退色及保护抗原的方法，在病理诊断中具有一定的实用价值。Objective To explore the different methods of decoloration for immunohistochemical HE slides, and their influence on tissue antigen expression. Methods Antigen retrieval solution （ EDTA or citric acid solution） was used in immunohistochemistry to decolor HE slides, and was compared with the method of hydrochloric acid solu- tion decoloration in normal temperature. Results Antigen heat retrieval decolors completely. Compared with other methods, it has shorter decoloration time （ 1 - 2min）, more than 90% of completely decoloration rate, the difference was significant（ x^2 = 27.6,23.9 ,all P 〈 0.01 ）. Meanwhile, the method dose not damage slides, and the immunohis- tochemical results are exact,without background coloring and unusual coloring, antigen expression rate and intensity are high. Conclusion Antigen heat retrieval decoloration not only simplifies the coloration process, but also protects tissue antigen activity better. It is the preferable method of decoloration and protecting antigen resource, which posses- ses some practical value in the pathology diagnosis.

关 键 词：免疫组织化学 染色与标记 实验室技术与方法 

分 类 号：R446.8[医药卫生—诊断学]