序贯培养对比序贯联合子宫内膜细胞共培养的方法对体外胚胎发育的影响  

Comparison of two methods ( “Sequential culture”& “Endometrial and Sequential co - culture”) on culturing discarded embryos in vitro

作　　者：晁贺[1] 刘英[1] 王树玉[1] 贾婵维[1] 任国庆[1] 余兰[1] 王丽[1] 李薇[1] 

机构地区：[1]首都医科大学附属北京妇产医院生殖中心,北京100026

出　　处：《中国优生与遗传杂志》2013年第9期96-98,F0002,共4页Chinese Journal of Birth Health & Heredity

基　　金：首都医科大学附属北京妇产医院院内基金项目编号:201114;北京市自然科学基金项目编号:5122015

摘　　要：目的观察序贯培养以及序贯联合子宫内膜细胞共培养两种方法,对辅助生殖技术中废弃胚胎的体外培养效果。方法收集常规体外受精IVF周期中的废弃胚胎,分别采用两种不同方法序贯培养法和联合培养法(序贯培养与子宫内膜细胞共培养)继续培养2-4天,观察胚胎发育情况,并用化学发光法检测两组囊胚形成后培养液中的HCG水平,比较囊胚形成能力。结果共收集46个废弃胚胎,序贯培养组23个废胚,形成囊胚3个,囊胚形成率13.04%(3/23),其中2个为优质囊胚(66.67%);联合培养组23个废胚,形成囊胚9个,囊胚形成率39.13%(9/23),其中8个为优质囊胚(88.89%)。联合培养组的囊胚形成率显著高于序贯培养组,有统计学差异(P<0.05);联合培养组的优质囊胚形成率高于序贯培养组,但无统计学差异(P>0.05)。联合培养组囊胚培养液中HCG水平为103.82±51.81mIU/L,序贯培养组为10.63±3.30mIU/L,前者高于后者,有统计学差异(P<0.05。)结论废弃胚胎体外培养仍能发育至囊胚阶段;序贯联合子宫内膜细胞共培养的方法,与单纯的体外序贯培养相比,其囊胚形成率更高,是进行废胚继续体外培养的更有效方法。Objective： To observe the effection of two methods （ ＂Sequential culture＂ ＆ ＂Endometrial and Sequential co- culture＂ ） in culturing discarded embryos in vitro during IVF - ET. Methods ： Discarded embryos from IVF/ICSI cycles were allocated to two groups randomly. In Group Sequential - culturing （ SC）, embryos were cultured only in Sequential medium, In Group Endometrial and Sequential co - cultureing （ ESC）, embryos were cocuhured on an endometrial stromal monolayer, meanwhile the Sequential medium was afford. All the embryos were cultured for another 2 - 4 days. Development of embryos was observed, and hCG level of the culture medium secreted by blastocysts was detected using a immunometric assay, to compare blastulation between the two groups. Results： Each group contains 23 discarded embryos. In Group Sequential - culturing, we got 3 blastocysts, the blastulation rate is 13.04%, 2 of them were quality ones （66. 67% ） ; and In Group Endometrial and Sequential co - cultureing, 9 blastocysts were got, the blastulation rate is 39. 13%, 8 blastocyst were quality ones （88.89%）. The blastulation rate is significantly increased in ESC compare with the SC, and blastocysts cultured in CG secreted higher levels of hCG compared with those cultured in SG （P 〈 0. 05 ）. However, there was no significant difference in the quality blastocyst formation rate between the two groups. Conclusion： Discarded embryos shows development potentiality to blastocyst; Endometrial combines Sequential medium offers a better development environment than only using Sequential medium during the In - vitro culture of discarded embryos.

关 键 词：胚胎 囊胚 子宫内膜细胞 序贯培养 共培养 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]