无裂栉江珧种内同工酶表型差异的比较研究  

A COMPARATIVE STUDY ON ISOZYME PHENOTYPIC DIVERGENCE AMONG FOUR TYPES OF PEN SHELL ATRINA PECTINATA LINNAEUS

作　　者：王梅芳[1] 余祥勇[1] 杨书婷[2] 桂建芳[2] 

机构地区：[1]湛江海洋大学水产学院,广东湛江524025 [2]中国科学院水生生物研究所淡水生态与生物技术国家重点实验室,湖北武汉430072

出　　处：《热带海洋》2000年第4期45-50,共6页

基　　金：国家重点实验室基金资助项目 (891 4B5 )

摘　　要：采用垂直板聚丙烯酰胺梯度凝胶电泳对 4种不同类型无裂栉江珧Atrinapectinata的肾脏、后闭壳肌和鳃组织进行了酯酶 (EST)、过氧化物歧化酶 (SOD)、乳酸脱氢酶 (LDH)、苹果酸脱氢酶 (MDH)、苹果酸酶 (ME) 5种同工酶的比较研究。结果表明这 4种类型的酶谱表达有差异 ,特别是肾组织的EST ,后闭壳肌的EST、SOD和鳃组织的SOD、MDH的酶谱表型差异明显 ,其特征谱带可作为区别无裂栉江珧种内差异的一种分子标记。The electrophoretograms of isozyme (EST, SOD, MDH, ME, LDH) from kidney, gill and muscle tissues of four types of pen shell Atrina pectinata Linnaeus were analyzed using vertical polyacrylamide gradient gel electrophoresis. The results suggested that there are obvious differences in the isozyme phenotypes of the four types, especially in EST isozyme from kidney, EST and SOD isozymes from muscle, SOD and MDH isozymes from gill. The characteristic electrophoretograms of isozyme were shown to be proper molecular markers to identify the four types of Atrina pectinata.

关 键 词：无裂栉江珧 表型差异 同工酶 分子标记 

分 类 号：Q959.215[生物学—动物学]