酵母铬对尼罗罗非鱼生长和糖代谢的影响  

Effects of Chromium Yeast on Growth and Glucose Metabolism of Nile Tilapia (Oreochromis niloticus)

作　　者：孙敏敏[1] 刘含亮[1] 王红卫[1] 孟晓[1] 王纪亭[1] 万文菊[2] 

机构地区：[1]山东农业大学动物科技学院,泰安271000 [2]泰山医学院,泰安271000

出　　处：《动物营养学报》2013年第9期2143-2149,共7页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：山东省自然科学基金项目(Y2008D35);山东省高等学校科技计划项目(J09LC05)

摘　　要：本试验旨在研究酵母铬对尼罗罗非鱼生长性能、血液生化指标、鱼体营养组成以及糖代谢相关基因胰岛素受体(insulin receptor,IR)和葡萄糖转运载体(glucose transporter,GLUT)表达的影响。选取平均体重为(15.80±0.02)g的尼罗罗非鱼300尾,随机分成4组,每组3个重复,每个重复25尾鱼。以酵母铬为铬源配制4种饲料,以三价铬(Cr3+)浓度计算,其添加水平分别为0(对照)、0.2、0.4和0.8 mg/kg。每组随机饲喂1种饲料。试验期为50 d。结果表明:虽然添加酵母铬对尼罗罗非鱼的生长性能、血液生化指标和鱼体营养组成的影响没有达到显著水平(P>0.05),也没有显著影响肝脏和肌肉中IR以及肌肉中GLUT4基因的表达(P>0.05),但0.8 mg/kg组肝脏中GLUT2基因表达量显著高于对照组和0.2 mg/kg组(P<0.05)。上述结果说明,添加酵母铬具有上调尼罗罗非鱼肝脏中GLUT2基因表达的潜在能力。The aim of this study was to evaluate the effects of chromium yeast on growth performance, blood biochemical indices, body nutrient composition and expression of insulin receptor （IR） and glucose transporter （GLUT） genes which related to glucose metabolism of Nile tilapia. Four diets were supplemented with 0 （control）, 0.2, 0.4 and 0.8 mg/kg trivalent chromium （Cr3＋） in the form of chromium yeast, respectively. A total of 300 Nile tilapia with an initial body weight of （15.80？0.02） g were randomly distributed into 4 groups with 3 replicates per group and 25 fish per replicate. Each diet was randomly assigned to one group. The experiment lasted for 50 days. The results showed that the chromium yeast supplementation did not significantly affect the growth performance, blood biochemical indices and body nutrient composition （P〉0.05）. The IR gene expression in liver and muscle, and the GLUT4 gene expression in muscle were not significantly affected by chromium yeast supplementation （P〉0.05）. However, the GLUT2 gene expression level in liver in the 0.8 mg/kg group was significantly higher than that in the control group and 0.2 mg/kg group （P〈0.05）. In summary, these results suggest that chromium yeast supplementation has a potential ability to improve the expression of GLUT2 gene in liver.

关 键 词：酵母铬 尼罗罗非鱼 生长性能 胰岛素受体 葡萄糖转运载体 

分 类 号：S963[农业科学—水产养殖]