GC测定苗药大果木姜子中1,8-桉叶素的含量  

Determination of 1,8-cineole in the Miao Ethnic Herb Cinnamomum migao H.W.Li by Gas Chromatography

作　　者：戴玮[1] 肖苏萍[1] 郭凯[1] 王继永[1] 赵润怀[1] 

机构地区：[1]中国药材公司,北京100195

出　　处：《中国现代中药》2013年第9期741-743,共3页Modern Chinese Medicine

基　　金：国家重大新药创制科技重大专项(2011ZX09102-003-05)

摘　　要：目的：采用气相色谱法建立大果木姜子中指标性成分1，8-桉叶素的含量测定方法。方法：AgilentDB-624毛细管（30in×0．25mm，1．4μm）色谱柱，载气为高纯氮气，进样口温度为200℃，检测器温度为250℃，程序升温：50℃保持5rain，5℃·min-1升温至150℃保持1min，10℃·min-1升温至250℃，保持20min。结果：1，8-桉叶素在0．3078～3．078mg·mL-1与峰面积呈良好线性关系，Y=364．28747X＋2．81698（r=0．9999），平均加样回收率为99．14％（RSD=3．14％）。结论：本方法操作简便、灵敏准确，可用于大果木姜子的质量控制。Objective： To establish a method of GC for the content determination of 1, 8-cineole in the miao ethnic herb Cinnamomum migao H. W. Li. Methods： The sample was separated on an Agilent DB-624（30 m × 0. 25 mm, 1.4 μm） fused silica capillary column with helium as the carrier gas. The inlet temperature was 200 ℃ and the FID temperature was 250℃. Temperature programming was started at 50 ℃, holding for 5min, then increased to 150 ℃ at a rate of 5 ℃·min-1. After holding for 1 min, increased to 250 ℃ at a rate of 10 ℃· min-1, holding for 20 min. Results： The calibration curve of 1, 8-cineole was linear in the range of 0. 307 8-3.078 mg · mL-1, r = 0. 999 9. The average recovery of 1, 8-cineole was 99. 14% （ RSD = 3.14% ） . Conclusion： The method was simple, sensitive, accurate, and could be used for the quality control of Fructus Cinnamomi.

关 键 词：1 8-桉叶素 大果木姜子 气相色谱 

分 类 号：R284.1[医药卫生—中药学]