人参内生真菌的分离及其抗肿瘤活性研究  

Isolation and Antitumor Activity Research of Endophytic Fungi from Panax ginseng

作　　者：王卓[1] 于慧美[2] 刘鼎[1] 高微[2] 田义新[1] 

机构地区：[1]吉林农业大学中药材学院,吉林长春130033 [2]吉林大学白求恩医学院,吉林长春130021

出　　处：《中国现代中药》2013年第9期748-751,共4页Modern Chinese Medicine

摘　　要：目的:从人参中分离获得人参内生真菌,并初步探讨其代谢产物粗提物的抗肿瘤活性。方法:研究利用筛选培养基从6年生人参中分离内生真菌,并利用小琼脂块培养法和分子生物学方法对获得的人参内生真菌进行鉴定,利用MTT法检测其体外抗肿瘤活性。结果:从人参中共计分离得到内生真菌28株,其中2株活性较好。结论:从药用植物人参中分离得到的人参内生真菌具有潜在的抗肿瘤活性,为进一步开发抗肿瘤药物提供新的研究对象。Objective： To isolate endophytic fungi from Panax ginseng and study their antitumor activity. Methods： Endophytic fungi were isolated from 6-year-old Panax ginseng. Their antitumor activities were analyzed by MTI＇ method in vitro. Results： 28 endophytic fungi were isolated from Panax ginseng, and 2 of them showed potential antitumor activity. Conclusion： Endophytic fungi isolated from Panax ginseng has antitumor activities, could be potential antitumor drugs.

关 键 词：人参 内生真菌 分离 生物活性 抗肿瘤 

分 类 号：R979.1[医药卫生—药品]