串珠镰孢D和E两个交配群电泳核型的研究  

Electrophoretic karyotype of mating population D and E of Fusarium moniliforme

作　　者：尚晓冬[1] 郑小波[1] 陆家云[1] 

机构地区：[1]南京农业大学农业部病虫监测与治理重点开放实验室,南京210095

出　　处：《南京农业大学学报》2000年第2期27-30,共4页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金 !(396 70 485 );高校博士点基金 !(B980 0 0 6 )资助项目

摘　　要：利用脉冲电泳技术研究了串珠镰孢D交配群和E交配群间及同一交配群内不同寄主和地区来源菌株电泳核型的相似性或多样性。结果显示 ,串珠镰孢D交配群菌株有 9～ 1 0条染色体 ,不同染色体DNA分子量变化范围为 0 7～ 6 1Mb ,基因组大小约为 33 3～ 37 9Mb ;E交配群供试菌株分离出 4～ 7条染色体 ,染色体DNA分子量变化范围为 0 7～ 4 8Mb ,部分基因组大小约为 9 0～ 2 1 4Mb。电泳核型分析结果表明 ,在串珠镰孢D和E两个交配群之间 ,供试菌株的电泳核型差异较大 ;两个交配群内部 ,供试菌株间电泳核型较相似 ,但仍显现出不同程度的差异。同一交配群菌株间电泳核型的差异与菌株的寄主来源和地区来源等无明显相关性。结果提示 。Electrophoretic karyotypes of mating population D and E of Fusarium moniliforme were obtained by using pulsed field gel electrophoresis(PFGE).9?10 chromosome sized DNA,varying from 0 7?6 1 Mb,were separated from the tested isolates of mating population D and estimated genome sizes were 33 3?37 9 Mb.For the mating population E,only 4?7 chromosome sized DNAs were separated,varying from 0 7?4 8 Mb,and the partial genome sizes were estimated to be 9 0?21 4 Mb.Isolates from the same mating population had similar electrophoretic karyotypes,regardless of their host of locality,but isolates from the different mating population had dissimilar electrophoretic karyotypes.

关 键 词：电泳核型 串珠镰孢 交配群D 交配群E 系统分类 

分 类 号：Q949.331[生物学—植物学] S432.44[农业科学—植物病理学]