雷帕霉素对基质细胞衍生因子-1α诱导的内皮生长晕细胞增殖及黏附能力的影响  

Rapamycin inhibits SDF-1α induced proliferation and adhesion of endothelial outgrowth cells

作　　者：蔡雪黎[1] 叶盛[2] 周希[1] 黄周青[1] 张怀勤[1] 黄伟剑[1] 

机构地区：[1]温州医科大学附属第一医院心内科,325000 [2]温州医科大学附属第二医院心内科

出　　处：《浙江医学》2013年第15期1399-1401,F0003,共4页Zhejiang Medical Journal

摘　　要：目的探讨雷帕霉素对基质细胞衍生因子-1α(SDF-1α)诱导的内皮生长晕细胞(EOCs)增殖及黏附能力的影响。方法使用密度梯度离心法分离脐血单个核细胞,培养并扩增EOCs,然后用免疫组化法、荧光染色法鉴定其内皮细胞特性。分为空白对照组、10ng/ml SDF-1α处理组、10ng/ml SDF-1α及10ng/ml雷帕霉素共同干预组、10ng/ml雷帕霉素处理组,各处理组与EOCs作用24h,CCK8检测细胞增殖能力,并在倒置显微镜下检测细胞的黏附能力。结果采用贴壁法培养的EOCs具有多种内皮细胞特性。10ng/ml的SDF-1α能显著提高EOCs的增殖及黏附能力(P<0.05),而10 ng/ml的雷帕霉素能阻断SDF-1α对EOCs增殖、黏附能力的促进作用(P<0.05)。结论雷帕霉素可抑制SDF-1α诱导的EOCs增殖及黏附。Objective To investigate the effects of rapamycin on SDF- 1α induced proliferation and adhesion of en-dothelial outgrowth cel （EOCs）. Methods The mononuclear cel s were harvested from umbilical cord blood by Ficol density gradient centrifugation, which were then induced into EOCs and expanded in vitro. The endothelial characteristics of EOCs were identified by immunostaining and fluorescent staining. The EOCs were divided into 4 groups： control group, SDF- 1α（10ng/ml） treatment group, rapamicin （10 ng/ml） treatment group and SDF- 1α＋rapamicin treatment group, the treatment in each group lasted for 24h. Proliferation of EOCs was evaluated by CCK8 and adhesion ability was measured under the inverted microscopy by counting adherent cel s after cel replacing. Results EOCs presented endothelial characteristics. Immunostaining showed that surface antigens Factor VIII, CD34 and Flk- 1 were positive; and fluorescent staining revealed that EOCs were bound to FITC- UEA- 1 and uptook DiI- ac- LDL. After a 24h- incubation, SDF- 1α （10 ng/ml） stimulated proliferation and adhesion activity of EOCs （P〈0.05）, while the effects were inhibited by rapamycin at the concentration of 10 ng/mL （P〈0.05）. Conclusion Ra-pamycin inhibits SDF- 1αinduced proliferation and adhesion of endothelial outgrowth cells.

关 键 词：雷帕霉素 基质细胞衍生因子-1Α 内皮生长晕细胞 再内皮化 血管狭窄 

分 类 号：R543.3[医药卫生—心血管疾病]