小麦远缘杂交后代花药培养最佳条件的研究  

Studies on anther culture of outbreeding progenies from triticum×triticale

作　　者：朱艳 汤剑力 吴根法 潘重光[2] 曹清玉[3] 沈大稜[3] 

机构地区：[1]上海宝山区生物技术中心,上海201907 [2]上海交通大学植物科学系,上海201101 [3]复旦大学遗传所,上海200433

出　　处：《上海农学院学报》2000年第2期138-141,共4页

基　　金：上海市科委科学基金资助项目!(993113027)

摘　　要：对远缘杂交后代花药培养条件的比较研究发现W14培养基比C17培养基更适用于小麦与小黑麦杂交后代的花药培养。花药培养的出愈率、再生率和绿苗率均和材料的遗传基础有一定的关系。培养中预处理温度和时间、初培养温度条件、花药密度效应、壮苗培养以及安全越夏都是提高花药培养效率的必要条件。Anther culture of outbreeding progenies from triticum×triticale was carried out on W14 and C17 medium.The data showed that W14 medium was more suitable to the anther culture than C17 medium.The ability to induce callus from anthers,plant regeneration and green plant regenerated was found to be species or genotype dependent.The essetial conditions for anther culture capacity are pretreatment temperature and time,culture temperature,anther density ,strong seedling culture and safely oversummering.

关 键 词：小麦 小黑麦 花药培养 远缘杂交 出苗 

分 类 号：S512.103.5[农业科学—作物学]