ITO玻璃芯片上温度分布对成肌细胞生长增殖的影响  

Impact of Temperature Profile on Indium-Tin Oxide Glass Chips on Growth and Proliferation of Myoblasts

作　　者：张晓娟[1] 杨忠[2] 王振宇[1] 胡宁[1] 黄小玲[1] 郑小林[1] 曹毅[3] 杨军[1] 

机构地区：[1]重庆大学生物工程学院,生物流变科学与技术教育部重点实验室,视觉损伤与再生修复重庆市重点实验室,重庆400030 [2]第三军医大学药学院,重庆400038 [3]重庆城市管理职业学院电子信息工程系,重庆400055

出　　处：《分析化学》2013年第8期1171-1176,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(Nos.81071278;31070882);教育部"新世纪优秀人才支持计划"(No.NCET-09-0842);中央高校基本科研业务费"研究生科技创新基金"项目(No.CDJXS11230046)资助

摘　　要：为了研究不同温度对小鼠成肌细胞生长增殖的影响,在ITO玻璃芯片上加载电场形成一定的温度分布。在不同温度(38、39、40和41℃)所对应的区域加工相同尺寸的PDMS微型培养腔室用于小鼠成肌细胞的培养。通过对芯片上培养的小鼠成肌细胞连续5 d的热刺激(30 min/d),研究不同温度短期热刺激对成肌细胞增殖的影响。细胞形态显微观察和流式细胞仪检测结果表明,一定的温度刺激对小鼠成肌细胞的增殖有促进作用,其中40℃刺激后的细胞数目增加最明显。在总刺激时间(30 min)相同情况下,短时多次热刺激的效果更理想,细胞增殖指数最高可达38.39。In order to study the impact of different temperatures on the growth and proliferation of myoblasts,specific electric fields were loaded on indium-tin oxide（ITO） glass chips to form some temperature profiles.In several zones with different temperatures（38,39,40 and 41 ℃）,microchambers with equal size were fabricated for the culture of mouse myoblasts.Impacts of temperatures on growth and proliferation of myoblasts were studied by stimulating cells for continuous 5 d（30 min/d）.Microscopic observation of cell morphology and flow cytometric detection results showed that certain thermal stimulation could promote the proliferation of myoblasts.Number of cells was obviously increased after the stimulus of 40 ℃.For equal total stimulation time,short-term multiple thermal stimulation could achieve better effect and maximum proliferation index could reach 38.39.

关 键 词：ITO玻璃 成肌细胞 热刺激 细胞周期 细胞增殖 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]