电喷雾萃取电离质谱直接检测人体呼出气体中的乙腈  

Direct Detection of Acetonitrile in Human Breath by Extractive Electrospray Ionization Mass Spectrometry

作　　者：张燕[1] 潘素素[1] 朱志强[1] 张兴磊[1] 徐高四[2] 魏益平[2] 陈焕文[1] 丁健桦[1] 

机构地区：[1]东华理工大学江西省质谱科学与仪器重点实验室,南昌330013 [2]南昌大学第二附属医院,南昌330006

出　　处：《分析化学》2013年第8期1220-1225,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(Nos.21265002;21105010);江西省科技支撑计划(Nos.2010BSA10200;2010DD01300)资助

摘　　要：人体呼出气体携带大量的生理病理信息,在临床诊断和代谢组学研究中发挥着日益重要的作用。本研究在未对样品进行预处理条件下,采用电喷雾萃取电离质谱(EESI-MS)技术直接测定呼出气体中乙腈分子。在优化的实验条件下,结合多级串联质谱,有效地获得了人体呼出气体中乙腈含量的信息。对气相乙腈的检出限为5.71 pL/L,线性范围为76.4～1910 pL/L。以两名健康志愿者呼出气体为实例,实际检测了其中乙腈的含量分别为134.49和104.40 pL/L,相对标准偏差(RSD)分别为6.3%和7.2%。结果表明,本方法灵敏度高、精密度好、分析速度快、特异性强,能够承受呼出气体中复杂基体的影响,适合于呼出气体中痕量乙腈的直接半定量检测,也可为呼出气体中其它小分子物质的检测提供新思路。Breath analysis by virtue of its potential medical research value,and as a non-invasive sample,simple sampling and continuous available is of interest for modern clinical diagnosis and metabonomics.The concentration levels of acetonitrile,an important biomarker in human exhaled gas,are correlated with human health.Herein we constructed a novel platform of extractive electrospray ionization-mass spectrometry（EESI-MS） for direct,rapid detection of acetonitrile in human breath without any sample pretreatment.Under the optimized working conditions,the quantitative information of acetonitrile in human breath was acquired effectively.For acetonitrile,a limit of detection was found to be 5.71 pL/L;the linear range was from 76.4 pL/L to 1910 pL/L.The concentration of acetonitrile in the exhaled breath of two healthy volunteers was in vivo determined to be 134.49 and 104.40 pL/L,respectively,the relative standard deviations were 6.3% and 7.2%.The results show that EESI-Ion Trap MS is a powerful analytical platform to provide high sensitivity,high precision,rapid analysis and high specificity for semi-quantitative analysis of acetonitrile in human breath,and for detection of other bio-molecules in exhaled breath potentially.

关 键 词：电喷雾萃取电离-质谱 乙腈 呼出气体 

分 类 号：R115[医药卫生—公共卫生与预防医学] O657.63[理学—分析化学]