绿竹花期生物学特性与愈伤组织诱导  

Floral Biology and Callus Induction of Bambusa oldhami

作　　者：姜可以[1] 张宁[1] 杨海芸[1] 桂仁意[1] 林新春[1] 方伟[1] 

机构地区：[1]浙江农林大学亚热带森林培育国家重点实验室培育基地,浙江临安311300

出　　处：《竹子研究汇刊》2013年第1期44-48,58,共6页Journal of Bamboo Research

基　　金：国家自然科学基金(31000295;31270677);浙江省自然科学基金重点项目(Z3100366)

摘　　要：为了建立绿竹高效愈伤组织诱导体系,研究了小穗的生物学特性,以及不同激素种类及浓度组合、单独添加2,4-D(2,4-Dichlorophenoxyacetic acid)浓度、有机添加物、甘露醇预处理等对小穗愈伤组织诱导的影响。结果表明:0.8～1 cm的小穗是诱导愈伤组织的最适宜材料;2,4-D与NAA组合小穗的愈伤组织诱导率最高,愈伤组织的质量也较好;小穗愈伤组织诱导以MS基本培养基添加3 mg·L-12,4-D、1 mg·L-1NAA和500 mg·L-1Proline、500 mg·L-1Glutamine、300 mg·L-1Casein Hydrolysate较好,愈伤组织诱导率最高,质量也相对较好;甘露醇预处理小穗愈伤组织出现时间推迟,愈伤组织质量与未处理的没有显著差异。To establish efficient system of callus induction, floral biology of Bambusa oldhami was studied. Meanwhile, the effects of external factors such as the combinations of different phytohormones, the concentrations of 2,4-Dichlorophenoxyacetic acid （2,4-D）, the supplement of organic additives and the mannitol pretreatment on callus induction of spikelets were tested. The results showed that the spikelets with 0.8 - 1 cm were the most appropriate for callus induction. The highest induction rate was achieved on MS medium supplemented with 2,4-D and NAA. Further, the basic MS medium added with 3 mg·L-1 2,4-D, 1 mg·L-1 NAA, 500 mg·L-1 Proline, 500 mg·L-1 Glutamine and 300 mg·L-1 Casein Hydrolysate was the best induction medium. The callus induction was delayed with the mannitol pretreatment, while the quality of callus was not improved.

关 键 词：绿竹 小穗 生物学特性 愈伤组织诱导 

分 类 号：S795.5[农业科学—林木遗传育种]