骨复活汤配合介入对兔SANFH股骨头局部VEGF表达的影响  

Effect of Gufuhuo Tang with Intervention on VEGF Expression of Local Femur Head in Rabbits with SANFH

作　　者：徐国华[1] 田伟明[1] 柴仪[1] 温志刚[1] 

机构地区：[1]河北省中医院,石家庄050011

出　　处：《河北中医药学报》2013年第3期3-4,2,共2页Journal of Hebei Traditional Chinese Medicine and Pharmacology

基　　金：河北省科技厅资助项目:No 11276169

摘　　要：目的:观察骨复活汤配合股动脉药物灌注治疗实验性SANFH股骨头局部VEGF的表达。方法:用Shwartzman反应和大剂量甲基强的松龙造成兔股骨头坏死模型,造模后第2周随机分为3组,介入组经左侧股动脉注射尿激酶和丹参注射液;骨复活汤配合介入组在介入组基础上,同时骨复活汤灌胃给药;对照组经股动脉注射生理盐水。在治疗后4周,对股骨头标本进行VEGF免疫组化染色。计数VEGF阳性成骨细胞率、阳性软骨细胞率和阳性血管率,并进行统计学分析。结果:骨复活汤配合介入组比介入组能提高VEGF阳性成骨细胞率、阳性软骨细胞率和阳性血管率,改善骨坏死。结论:骨复活汤配合介入能改善骨坏死,其机理在于促进VEGF等细胞因子的分泌从而加速股骨头的再血管化和再骨化进程。Objective: to observe the effect of Gufuhuo Tang on VEGF expression of local femur head in treating SANFH combined with drug perfusion in femoral artery. Methods: Shwartzman reaction and high-dose methylprednisolone were used to produce the rabbit model with femur head necrosis,which,one week later,were randomly divided into three groups: intervention group injected with urokinase and Danshen injection to the left femoral artery; Gufuhuo Tang group given intragastric administration as well as intervention; control group injected with physiological saline to the femoral artery. Four weeks after treatment,the samples were given immunohistochemisty dyeing. Count VEGF positive osteoblasts rate,positive cartilage rate and positive blood vessels rate,and make statistical analyses. Results: The three rates of Gufuhuo Tang group were all higher than those of the intervention group,and osteonecrosis was also improved. Conclusion: Gufuhuo Tang is able to improve osteonecrosis,the mechanism of which lies in promoting VEGF secretion so as to accelerate the revascularization and reossification of the femur head.

关 键 词：骨复活汤 股动脉 激素性股骨头缺血性坏死(SANFH) 血管内皮细胞生长因子(VEGF) 

分 类 号：R285.5[医药卫生—中药学]