二色桌片参的化学成分研究 Ⅳ 二色桌片参糖蛋白的分离性质与抗肿瘤活性的研究  

STUDIES ON THE CHEMICAL CONSTITUENTS FROM SEA CUCUMBER MENSAMARIA INTERCEDENS Ⅳ ISOLATION,PROPRETIES AND ANTITUMOR ACTIVITY OF THE GLYCOPROTEIN FROM MENSAMARIA INTERCEDENS

作　　者：吴萍茹 陈粤[2] 方金瑞 苏文金[3] 

机构地区：[1]福建省海洋研究所,厦门361012 [2]汕头大学理学院生物学系,汕头515063 [3]厦门大学细胞生物学与肿瘤细胞工程教育部重点实验室,厦门361005

出　　处：《中国海洋药物》2000年第5期4-6,共3页Chinese Journal of Marine Drugs

基　　金：福建省重中之重项目资助

摘　　要：二色桌片参〔Mensamaria intercedens( L ampert)〕的体壁经水煮提、分离、最后经 Sephadex G- 2 0 0柱层析纯化得二色桌片参糖蛋白 ( GPMI- )。GPMI- 经酶解后得二色桌片参糖蛋白 ( GPMI- )。分别研究了GPMI- 和 GPMI- 的性状、化学组成和抗肿瘤活性 ,结果表明 :GPMI- 和 GPMI- 都是糖蛋白 ,但蛋白组成和糖含量明显不同。GPMI- 对小鼠 S180 肉瘤有明显抑制作用 ,而无直接毒性 ,抑瘤率为 4 8.8%。而 GPMI- 却能显著提高 S180 肉瘤荷瘤小鼠的脾指数 ,呈免疫增强作用。Glycoprotein of Mensamaria intercedens(GPMI Ⅰ) was extracted from the body of Mensamaria intercedens with hot water,and purification was finished with column chromatography on Sephadex G 200. GPMI Ⅰ was hydrolyzed with enzymes to obtain glycoprotein of Mensamaria intercedens Ⅱ(GMI Ⅱ).The chemical property,composition and antitumor activity of GPMI Ⅰ and GPMI Ⅱ were studied. The results showed that GPMI Ⅰ and GPMI Ⅱ were two kinds of glycoproteins in which the content of protein and sugar were different. GPMI Ⅱ could significantly inhibit the growth of Sarcoma 180 cells implanted subcutaneously in mice (P<0.05) at the dose of 20 mg·kg -1 per day ×10 with no sign of toxicity. The ratio of tumor inhibition is 48.8%. On the other hand, GPMI Ⅰ increased the weight of spleen of mice with S 180 cells at the dose of 30 mg·kg -1 per day×10.

关 键 词：糖蛋白 二色桌片参 抗肿瘤活性 化学成分 

分 类 号：R282.77[医药卫生—中药学] R979.1[医药卫生—中医学]