黄芪对Lewis肺癌细胞核形态、DNA含量及AgNORs计数的影响  

Effect of Astragalus on Nuclear Morphometry,DNA Contents and AgNORs Counting of Lewis Lung Cancer

作　　者：胡雪峰[1] 刘铭球[1] 陈觅[2] 江曼[1] 刘绚[1] 李蓓云[1] 夏东[1] 邹祖玉[1] 

机构地区：[1]武汉大学,武汉430071 [2]武汉武警湖北总队医院

出　　处：《中国中西医结合外科杂志》2000年第5期319-321,共3页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：湖北省自然科学基金!( 94J0 80 );湖北省科委重点科研课题!( 962P110 6)

摘　　要：目的 :探讨黄芪对Lewis肺癌的抑制作用。 方法 :将Lewis肺癌细胞接种于C57BL/6小鼠皮下 (1.2 5× 10 6/鼠 )。将 2 0只小鼠随机分为Ⅰ、Ⅱ组 ,接种后第 5d分别给予生理盐水、黄芪 ,第 18d处死动物。利用图像分析仪对肿瘤细胞核形态、DNA含量及AgNORs计数进行测量。 结果 :Ⅱ组核面积 (2 2 .2 5 9± 4.972 ) μm2 、周长 (17.943± 2 .45 7) μm、平均直径 (2 .6 46±0 .2 94) μm、积分光密度 3.35 8± 0 .85 6、AgNORs计数 5 .0 38± 0 .815比Ⅰ组小 ,差异均有显著性 (P <0 .0 5 )。 结论 :黄芪对Lewis肺癌具有抑制作用。Objective:To investigate the inhibiting effects of astragalus root on Lewis lung cancer(LLC).Methods:Lewis lung cancer cells(1.25×10 6/mouse)were inoculated subcutaneously to 20 mice,that were divided randomly into 2 groups.From the 5th day of inoculation,normal saline and astragalus solution were injected respectively.On the 18th day,all the mice were killed,nuclear morphometry,DNA contents and AgNORs counting were detected by image analysis.Results:Nuclear area,perimeter,mean diameter,DNA contents,AgNORs counting in group Ⅱ are smaller than in group Ⅰ(P<0.05).Conclusion:astragalus root can inhibit LLC.

关 键 词：肺肿瘤 黄芪 DNA含量 细膜胲 AGNORS 

分 类 号：R273.42[医药卫生—中西医结合] R282.710.5[医药卫生—中医肿瘤科]