电穿孔技术促进盐酸丁卡因经皮渗透的研究  

Transdermal delivery of tetracaine hydrochloride by electroporation

作　　者：胡巧红[1] 梁文权 包家立[2] 平其能[3] 

机构地区：[1]浙江大学湖滨校区药学院药剂教研室,浙江杭州310006 [2]浙江大学临床医学工程研究所,浙江杭州310006 [3]中国药科大学药学院,江苏南京210009

出　　处：《中国药学杂志》2000年第10期673-676,共4页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目!( 3 9670 85 7)

摘　　要：目的　研究电穿孔技术对小分子离子型药物经皮渗透的影响。方法　以盐酸丁卡因为小分子离子型模型药物 ,使用双室扩散池方法研究电穿孔对其在离体大鼠腹部皮肤经皮渗透的影响 ,并与被动扩散和离子导入进行比较。结果　外加电脉冲 (方波脉冲 ,脉冲幅度为 130V ,脉冲宽度为 0 .4s,脉冲率为 40pulses·min-1)后 ,盐酸丁卡因的渗透速率较被动扩散增加 3.5倍以上 ,比离子导入 (0 .2mA·cm-2 ,4h)增加 1倍以上 ;并且渗透速率随着脉冲数的增加而增加。结论　电穿孔技术能明显地促进小分子离子型药物盐酸丁卡因的经皮渗透。OBJECTIVE To study the effects of electroporation on the permeation of small molecular ion drug through skin in vitro.METHODS Using valia chien two chamber diffusion cells,the fluxes of tetracaine hydrochloride achieved with passive diffusion, electroporative pulse and iontophoresis were compared.RESULTS The application of electroporative pulse or iontophoresis increased the permeation of tetracaine hydrochloride. The flux of tetracaine hydrochloride with electroporative pulse (square wave pulse, voltage=130 V, pulse time=0.4 s,pulse freqency=40 pulses·min -1 ) was 4 times or 2 times higher than that of passive diffusion or iontophoresis (0.2 mA·cm -2 , 4 h),respectively.When the pulse number was increased, the flux of tetracaine hydrochloride was increased.CONCLUSION The permeation of tetracaine hydrochloride through skin can be increased by electroporation.

关 键 词：电穿孔 经皮渗透 盐酸丁卡因 离子导入 

分 类 号：R971.2[医药卫生—药品] R944.9[医药卫生—药学]