盐酸氨溴索防治早期放射性肺损伤的实验研究  

Preventive and therapeutic effects of ambroxol hydrochloride on prophase of radiation-induced lung injury in mice

作　　者：张洁旻[1] 张纬建[2] 陈秀英[2] 许建华[3] 

机构地区：[1]福建医科大学附属第一医院药学部,350005 [2]福建医科大学附属第一医院放疗科,350005 [3]福建医科大学药学院

出　　处：《中国现代药物应用》2013年第19期1-3,共3页Chinese Journal of Modern Drug Application

摘　　要：目的探讨盐酸氨溴索对小鼠早期放射性肺损伤的防治作用。方法成年雄性昆明种小鼠36只,随机分成对照组,模型组和盐酸氨溴索组,每组12只。对照组小鼠无照射无给药,模型组灌胃生理盐水10 ml/(kg·d),盐酸氨溴索组灌胃盐酸氨溴索口服溶液40 mg/(kg·d),连续5 d。第5天给药后1 h,模型组和盐酸氨溴索组小鼠全肺单次照射18 Gy X射线,照射后继续给药3周。于照射后2周、6周,进行小鼠肺部螺旋CT扫描及图像分析,获得小鼠全肺的平均灰度值;采用HE染色,制作肺组织病理切片,在光镜下观察肺组织的变化。结果盐酸氨溴索组小鼠全肺平均灰度值明显低于模型组(P<0.05);模型组小鼠在照射后存在明显的病理组织学上的改变,表现为肺充血、水肿、肺间质增厚,盐酸氨溴索组上述所见明显减轻。结论盐酸氨溴索对放射性肺损伤有一定的防治作用。Objective To investigate the preventive and therapeutic effects of ambroxol hydrochloride in the early time of radiation-induced lung injury in mice. Methods Thirty six Kunming mice were randomly classified into 3 groups ： a control group, a treatment group and a ambroxol hydrochloride group. Control group received neither radiation nor ambroxol hydrochloride; Treatment group was orally administrated natural saline 10 ml/（kg.d） and ambroxol hydrochloride group was orally administrated ambroxol hydrochloride 40 mg/（kg · d） for 5 days. The whole thorax of treatment group and ambroxol hydrochloride group mice were irradiated with 18 Gy X-Ray one hour after administer in the fifth day, intragastric administration were kept for 3 weeks after irradiation. CT scanning and image analysis of the mean gray value were carried out to acquire the data of each mouse after irradiation for 2 and 6 weeks. The pathologic changes of lung were studied by the way of HE staining pathologic of lung. Results Compared with treatment group ,the mean gray value of ambroxol hydrochloride group obviously decreased （P〈0.05）; significant histological and pathologic changes were observed in treatment group, while above injuries were obviously slight in ambroxol hydrochloride group. Conclusion Ambroxol hydrochloride have the preventive and therapeutic effects in the early time of radiation-induced lung injury.

关 键 词：盐酸氨溴索 放射性肺损伤 图像分析 

分 类 号：R818[医药卫生—放射医学]